Cofilin (D3F9) XP? Rabbit mAb

• 产品编号：CST-5175P      品牌：Cell Signaling Technology       原厂货号：5175P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cofilin (D3F9) XP^® Rabbit mAb识别内源性总的cofilin1蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人源cofilin1蛋白邻近中心的氨基酸序列一致。Cofilin和actin-解聚因子（ADF）都属于一组保守的小actin-结合蛋白家族，在胞质分裂，内吞，胚胎发育，压力应激和组织再生过程中发挥了关键作用（1）。在刺激因素作用下，cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生（2）。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用（3-5）。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中（6）。

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

原厂资料：

Specificity / Sensitivity

Cofilin (D3F9) XP® Rabbit mAb detects endogenous levels of total cofilin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein.

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody