GAPDH (D16H11) XP? Rabbit mAb

• 产品编号：CST-5174P      品牌：Cell Signaling Technology       原厂货号：5174P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GAPDH (D16H11) XP® Rabbit mAb兔单抗用于检测内源性GAPDH总蛋白水平。该单抗是通过合成对应于人GAPDH蛋白羧基端序列的肽段免疫动物获得甘油醛－3－磷酸脱氢酶（GAPDH）在糖酵解过程中将3-磷酸甘油醛进一步磷酸化。虽然在不同组织中的表达水平有差异，但GAPDH普遍被认为是一个组成型表达的持家蛋白。正是由于这个原因，GAPDH的mRNA和蛋白通常在对其它有变化的靶蛋白测定实验时作为对照。最近的研究阐明了GAPDH在细胞凋亡（2），基因表达（3）和核转运（4）过程中的作用。GAPDH还可能对神经退行性疾病如亨廷顿和阿尔茨海默病的发生起着作用（4,5）。

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

GAPDH (D16H11) XP® Rabbit mAb detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAPDH. 　

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer''s diseases (4,5).

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody