Phospho-CD79A (Tyr182) Antibody

• 产品编号：CST-5173S      品牌：CST       原厂货号：5173S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CD79A (Tyr182)能够检测在Tyr182位点磷酸化的内源CD79A蛋白。此多克隆抗体是通过合成小鼠源对应的CD79A Tyr182位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。此多克隆抗体是通过合成小鼠源对应的CD79A Tyr182位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。CD79A的Tyr182位点能够被Src家族激酶Lyn和Blk磷酸化(10)。

1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
9. Vuillier, F. et al. (2005) Blood 105, 2933-40.
10. Pao, L.I. et al. (1998) J Immunol 160, 3305-14.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-CD79A (Tyr182) Antibody detects endogenous levels of CD79A protein only when phosphorylated on Tyr182 of CD79A.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr182 of Mouse CD79A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Antigen receptors found on the surface of B-cells contain a hetrodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) is found in the CD79A intracellular region appears to be is important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). CD79A is a marker for B-lineage lymphoblastic leukemia (8); mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia (9).Tyr182 of CD79A can be phosphorylated by Src family kinases Lyn and Blk (10).

1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
9. Vuillier, F. et al. (2005) Blood 105, 2933-40.
10. Pao, L.I. et al. (1998) J Immunol 160, 3305-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.