EAPP (1E4) Mouse mAb

• 产品编号：CST-5166S      品牌：CST       原厂货号：5166S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EAPP (1E4) Mouse mAb 能够检测内源性EAPP总蛋白。该单克隆抗体是由重组的人EAPP蛋白氨基末端片段免疫动物生产的。E2F转录因子家族的调节主要通过pRb，该过程对于细胞增殖和细胞死亡至关重要(回顾1)。E2F相关的磷蛋白(EAPP)被鉴定为E2F家族结合蛋白，能够调节E2F调控的转录并可能参与S期的进入。EAPP在所有的组织和细胞中表达各不相同，而且其水平在诺考达唑捕获的细胞中是降低的(2)。质谱研究已经鉴定出EAPP的多个磷酸化位点包括109位和111位的丝氨酸，但是EAPP磷酸化的生物学作用还未被阐明(3-5)。

1. Polager, S. and Ginsberg, D. (2008) Trends Cell Biol 18, 528-35.
2. Novy, M. et al. (2005) Mol Biol Cell 16, 2181-90.
3. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
4. Mayya, V. et al. (2009) Sci Signal 2, ra46.
5. Olsen, J.V. et al. (2006) Cell 127, 635-48.

原厂资料：

Specificity / Sensitivity

EAPP (1E4) Mouse mAb detects endogenous levels of total EAPP protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant amino-terminal fragment of human EAPP protein. 

Background

Regulation of the E2F family of transcription factors, primarily through the retinoblastoma protein (pRb), is vital for control of cell proliferation and cell death (reviewed in 1). E2F-associated phosphoprotein (EAPP) was identified as an E2F-family binding protein that modulates E2F-regulated transcription and may be required for S phase entry. EAPP is expressed at varied levels in all tissues and cell types examined and its expression is reduced in nocodazole-arrested cells (2). Mass spectrometry studies have identified multiple EAPP phosphorylation sites including Ser109 and Ser111, but biological consequences of EAPP phosphorylation have yet to be elucidated (3-5). 

1. Polager, S. and Ginsberg, D. (2008) Trends Cell Biol 18, 528-35.
2. Novy, M. et al. (2005) Mol Biol Cell 16, 2181-90.
3. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
4. Mayya, V. et al. (2009) Sci Signal 2, ra46.
5. Olsen, J.V. et al. (2006) Cell 127, 635-48.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.