Human Interleukin-28A (hIL-28A/IFN-λ2)

• 产品编号：CST-5164LF      品牌：CST       原厂货号：5164LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的人重组蛋白IL-28A (hIL-28A) Val26-Val200 (Accession #NP_742150)是从人的293细胞表达而来。基于氨基酸测序的基础上，多于60%的重组hIL-28A 蛋白在Val26 (VPVAR) 起始表达，分子量为19,754Da。其余的在Pro27 (PVARL)或Val28 (VARLH)表达。 DTT还原的和未还原的蛋白作为21 kDa 多肽而迁移。6 μg 还原 (+) 和 非还原的(-) 重组hIL-28A通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hIL-28A的生物活性是通过病毒保护实验来确定的。每个批次的ED50在10-60 ng/ml之间。每微克hIL-28A内毒素含量小于0.01 ng。

有载体：每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIFN-γ蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-28B是干扰素λ细胞因子家族成员之一，包括IL-29和IL-28A(1)。IL-28B在很多类型的细胞中产生并且与参与类型I干扰素的很多功能(1-3)。IL-28B在体内和体外都存在抗病毒的活性(1,2)。很多的研究表明，IL-28B在体内直接抑制肿瘤细胞的增殖并促进抗肿瘤免疫反应(1,3)。IL-28的受体是包含IL-28Rα和 IL-10RI的异源二聚体(1)。IL-28A激活Stat1、Stat3 和Stat5 (1)。IL-28Rα的表达只是局限在一小部分的细胞类型中，包括类浆细胞DC 和上皮细胞(1)。

原厂资料：

Source / Purification

Recombinant human IL-28A (hIL-28A) Val26-Val200 (Accession #NP_742150) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 60% of recombinant hIL-28A starts at Val26 (VPVAR) and has a calculated MW of 19,754. The remainder starts at Pro27 (PVARL) or Val28 (VARLH). DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-28A. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-28A was determined in a virus protection assay. The ED50 of each lot is between 10-60 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-28A. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-28A is a member of the Interferon λ family of cytokines, which includes IL-29 and IL-28B (1). IL-28A is produced by a number of cell types and shares many functions with Type I Interferons (1-3). IL-28A exhibits anti-viral activities in vitro and in vivo (1,2). IL-28A directly inhibits tumor cell proliferation and promotes anti-tumor immune responses in vivo (1,3). The IL-28 receptor is a heterodimer of the IL28-Rα and IL10-RI (1). IL-28A activates Stat1, Stat3, and Stat5 (1). IL-28Rα expression is limited to a few cell types, including plasmacytoid DC and epithelial cells (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.