Phospho-ATRIP (Ser224) Antibody

• 产品编号：CST-5161S      品牌：CST       原厂货号：5161S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-ATRIP (Ser224)抗体能够检测内源性丝氨酸(224位点)磷酸化的ATRIP蛋白。该多克隆抗体是由合成的人源的针对ATRIP蛋白近丝氨酸(224位)的磷酸化肽段免疫动物生产的。在基因组应激中，ATR相互作用蛋白(ATRIP)结合并被DNA损伤和检验点激活激酶ATR(共济失调毛细血管扩张症兼Rad3相关激酶)磷酸化。ATR和ATRIP在检验点信号通路中是一体的并在DNA修复反应中起重要作用(1-3)。RPA包被的单链DNA处ATRIP和RPA (复制蛋白A)的直接相互作用引起磷酸化ATR/ATRIP招募从而导致复制叉停滞和DNA损伤(3)。ATR/ATRIP协同重要的调节蛋白如Rad17 和9-1-1 复合物调整DNA修复和细胞周期进程(4)。TOPBP1(拓扑异构酶II结合蛋白)可以激活ATR与ATRIP的相互作用，这表明ATRIP可能调节ATR的定位和活性(5)。细胞周期依赖性蛋白激酶2(CDK2)可通过ATRIP蛋白丝氨酸(224位点)磷酸化参与DNA损伤反应和细胞周期控制的调节。

1. Cortez, D. et al. (2001) Science 294, 1713-1716.
2. Itakura, E. et al. (2004) Biochem. Biophys. Res. Commun. 323, 1197-1202.
3. Zou, L. and Elledge, S.J. (2003) Science 300, 1542-1548.
4. Yang, X.H. and Zou, L. (2006) Methods Enzymol. 409, 118-131.
5. Ball, H.L. et al. (2007) Mol Cell Biol 27, 3367-77.
6. Myers, J.S. et al. (2007) Cancer Res 67, 6685-90.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey

Specificity / Sensitivity

Phospho-ATRIP (Ser224) Antibody detects endogenous levels of ATRIP protein only when phosphorylated at Ser224. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phoshopeptide corresponding to residues surrounding Ser224 of the human ATRIP protein. 

Background

In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5). 

Cyclin dependent kinase 2 (CDK2) may participate in the regulation of DNA damage response and cell cycle control through phosphorylation of ATRIP at Ser224 (6).

1. Cortez, D. et al. (2001) Science 294, 1713-1716.
2. Itakura, E. et al. (2004) Biochem. Biophys. Res. Commun. 323, 1197-1202.
3. Zou, L. and Elledge, S.J. (2003) Science 300, 1542-1548.
4. Yang, X.H. and Zou, L. (2006) Methods Enzymol. 409, 118-131.
5. Ball, H.L. et al. (2007) Mol Cell Biol 27, 3367-77.
6. Myers, J.S. et al. (2007) Cancer Res 67, 6685-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody