Frizzled6 (D16E5) Rabbit mAb

• 产品编号：CST-5158S      品牌：CST       原厂货号：5158S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Frizzled6 (D16E5)兔单抗检测内源的总Frizzled6 蛋白。单克隆抗体由合成的肽段免疫动物产生，该肽段与人源Frizzled6 蛋白的羧基末端的氨基酸残基序列一致。Frizzled (Fzd)属于七大跨膜转移G蛋白偶联受体(GPCR)超家族(1)。Frizzled有一个巨大的胞外N末端区域，含有一个胱氨酸富集的结构域（CRD），这些结构与其结合Wnt蛋白有关（1,2）。细胞内C末端与Dvl 蛋白的PDZ结构域结合，Dvl是Fzd下游主要信号通路元件(3)。Wnt蛋白结合Fzd和共受体LRP5和LRP6，通过抑制GSK3-β对β-catenin的磷酸化来激活Wnt/β-catenin 通路 (4,5)。除经典Wnt/β-catenin通路，一些Wnt蛋白也能激活Fzd/Ca2+ 和 Fzd/PCP9(平面细胞极性)通路[6,7],哺乳动物Fzd亚家族由十个成员（(Fzd1到Fzd10），它们可能通过不同通路调控下游信号(8)。一些Fzd也能和其他分泌蛋白结合，如Norrin和R-Spondin(9-11).

1. Schulte, G. and Bryja, V. (2007) Trends Pharmacol Sci 28, 518-25.
2. Hsieh, J.C. et al. (1999) Proc Natl Acad Sci USA 96, 3546-51.
3. Wong, H.C. et al. (2003) Mol Cell 12, 1251-60.
4. Zeng, X. et al. (2005) Nature 438, 873-7.
5. Davidson, G. et al. (2005) Nature 438, 867-72.
6. Fanto, M. and McNeill, H. (2004) J Cell Sci 117, 527-33.
7. Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46.
8. Cadigan, K.M. and Liu, Y.I. (2006) J Cell Sci 119, 395-402.
9. Xu, Q. et al. (2004) Cell 116, 883-95.
10. Nam, J.S. et al. (2006) J Biol Chem 281, 13247-57.
11. Hendrickx, M. and Leyns, L. (2008) Dev Growth Differ 50, 229-43.

原厂资料：

Specificity / Sensitivity

Frizzled6 (D16E5) Rabbit mAb detects endogenous levels of total Frizzled6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Frizzled6 protein.

Background

Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11).

1. Schulte, G. and Bryja, V. (2007) Trends Pharmacol Sci 28, 518-25.
2. Hsieh, J.C. et al. (1999) Proc Natl Acad Sci USA 96, 3546-51.
3. Wong, H.C. et al. (2003) Mol Cell 12, 1251-60.
4. Zeng, X. et al. (2005) Nature 438, 873-7.
5. Davidson, G. et al. (2005) Nature 438, 867-72.
6. Fanto, M. and McNeill, H. (2004) J Cell Sci 117, 527-33.
7. Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46.
8. Cadigan, K.M. and Liu, Y.I. (2006) J Cell Sci 119, 395-402.
9. Xu, Q. et al. (2004) Cell 116, 883-95.
10. Nam, J.S. et al. (2006) J Biol Chem 281, 13247-57.
11. Hendrickx, M. and Leyns, L. (2008) Dev Growth Differ 50, 229-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.