HGK (D19H10) Rabbit mAb

• 产品编号：CST-5146S      品牌：CST       原厂货号：5146S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HGK (D19H10) Rabbit mAb兔单抗能检测内源性总HGK蛋白的水平。该单克隆抗体通过使用与人源HGK蛋白丙氨酸（553位点）周围残基相一致的合成肽段免疫动物而获得。HGK (MAP4K4 或有丝分裂原活化的蛋白激酶4）是一种丝氨酸/苏氨酸激酶，属于哺乳动物 STE20/MAP4K激酶家族。它参与应答环境应激反应和细胞因子如TNF-α (1-3)。HGK特异性活化c-Jun N-terminal kinase (JNK)信号传导途径和提高体内AP-1-调节的转录活性 (1)。HGK广泛表达与各种类型的人类癌症和癌症细胞系，并在细胞转型、入侵、粘附和迁移过程中起着重要作用(4,5)。

1. Yao, Z. et al. (1999) J Biol Chem 274, 2118-25.
2. Tesz, G.J. et al. (2007) J Biol Chem 282, 19302-12.
3. Bouzakri, K. and Zierath, J.R. (2007) J Biol Chem 282, 7783-9.
4. Wright, J.H. et al. (2003) Mol Cell Biol 23, 2068-82.
5. Collins, C.S. et al. (2006) Proc Natl Acad Sci USA 103, 3775-80.

原厂资料：

Specificity / Sensitivity

HGK (D19H10) Rabbit mAb detects endogenous levels of total HGK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala553 of human HGK protein.

Background

HGK (MAP4K4 or mitogen-activated protein kinase kinase kinase kinase 4) is a serine/threonine kinase that belongs to the mammalian STE20/MAP4K kinase family involved in response to environmental stress and cytokines such as TNF-α (1-3). HGK specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway and increases AP-1-mediated transcriptional activity in vivo (1). HGK is broadly expressed in many types of human cancer and cancer cell lines and plays an important role in cell transformation, invasiveness, adhesion and migration (4,5).

1. Yao, Z. et al. (1999) J Biol Chem 274, 2118-25.
2. Tesz, G.J. et al. (2007) J Biol Chem 282, 19302-12.
3. Bouzakri, K. and Zierath, J.R. (2007) J Biol Chem 282, 7783-9.
4. Wright, J.H. et al. (2003) Mol Cell Biol 23, 2068-82.
5. Collins, C.S. et al. (2006) Proc Natl Acad Sci USA 103, 3775-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.