Myosin II Isoform Antibody Sampler Kit

• 产品编号：CST-5144S      品牌：Cell Signaling Technology       原厂货号：5144S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Myosin II Isoform Antibody Sampler Kit中每个抗体检测内源性特异的myosin II亚型总蛋白。通过人工合成小鼠myosin IIa蛋白和人源myosin IIb和IIc蛋白羧基末端周围相应的多肽片段去免疫动物从而制备出多克隆抗体。Myosin II Isoform Antibody Sampler Kit是一个经济的方法去检测myosin II亚型a、b和c总蛋白水平。该试剂盒有充足一抗和二抗去进行四次免疫印迹（Western Blot）实验。非肌性myosin蛋白是一个肌动蛋白为基础的马达蛋白，其对细胞运动、细胞分裂、迁移、粘附和极化有着本质作用。全酶由两个完全相同的重链和两种轻链组成。轻链(MLCs)调节myosin II蛋白的活性和稳定。重链(NMHCs)通过三个基因MYH9、MYH10和MYH14编码，这就分别产生三种不同的非肌性myosin II亚型 IIa, IIb, and IIc（1）。当三个亚型进行同样的酶促任务时，它们结合到并收缩耦合了ATP水解作用的肌动蛋白纤维，它们的细胞内功能没有出现过剩，并且它们有不同的亚细胞分布(2-5)。myosin II蛋白羧基端尾部结构域在亚型特异性亚细胞定位中有重要作用 (6)。myosin IIa蛋白在Ser1943位点的磷酸化有助于乳腺癌细胞转移的调节(7)。

1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

原厂资料：

Specificity / Sensitivity

Each antibody in the Myosin II Isoform Antibody Sampler Kit detects endogenous total levels of the specified myosin II isoform.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the carboxy terminus of mouse myosin IIa and human myosin IIb and IIc.

Description

The Myosin II Isoform Antibody Sampler Kit is an economical way to examine the total protein levels of myosin II isoforms a, b, and c. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.

Background

Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.