MYPT1 Antibody Sampler Kit

• 产品编号：CST-5143S      品牌：Cell Signaling Technology       原厂货号：5143S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

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描述：

通过人工合成人源MYPT1蛋白Ser507、Ser668、Thr853和Thr696位点周围相应的磷酸化片段去免疫动物从而制备出磷酸化特异性多克隆抗体。通过人工合成人源MYPT1蛋白氨基末端周围相应的多肽片段去免疫动物从而制备出MYPT1 Antibody。通过蛋白A和多肽亲和层析纯化抗体。 MYPT1 Antibody Sampler Kit是一个经济方法去检测MYPT1总蛋白和在Ser507、Ser668、Thr853和Thr696磷酸化的MYPT1蛋白。该试剂盒含有充足的一抗和二抗去进行四次免疫荧光实验。Protein phosphatase 1 (PP1)是一个普遍存在的真核蛋白质丝氨酸/苏氨酸磷酸酶，其涉及不同细胞功能的调节。底物的特异性通过一个调节亚单位与PP1 catalytic subunit (PP1c)的结合被确定。据估计大约有五十个不同的调节亚单位存在(1)。myosin 磷酸酶全酶有三个亚单位组成：PP1催化亚单位(PP1c) 、一个靶向/调节亚单位（MYPT/myosin-binding subunit of myosin phosphatase）和一个未知功能的20 kDa大小的亚单位 (M20)。MYPT蛋白结合到PP1cδ蛋白可改变催化部位的构象以及子女国家酶活性和特异性 (2)。61%序列相同的两个MYPT亚单位已经被描述。MYPT1蛋白广泛表达，而MYPT2 蛋白的表达只表达在心脏和大脑组织(3)。相关的家族成员也诱导MBS85、MYPT3和TIMAP (4)。在小GTPase Rho的信号下，Myosin磷酸酶调节actin和myosin相互作用。通过Rho-associated kinase (ROCK)作用，Rho活性抑制myosin磷酸酶。MYPT1蛋白在Thr696和Thr853位点的磷酸化导致磷酸酶抑制和细胞骨架重组(5,6)。Phospho-MYPT1 (Ser507)和phospho-MYPT1 (Ser668) antibodies直接作用的位点，该位点磷酸化被鉴定 ，通过Cell Signaling Technology (CST)公司使用PhosphoScan®, a CST™ LC-MS/MS平台发现修饰的位点(5)。这些位点的磷酸化使用一个Akt底物抗体被发现。请访问PhosphoSitePlus®, CST's修饰位点知识库，更多的信息查询网站：www.phosphosite.org。

1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
2. Terrak, M. et al. (2004) Nature 429, 780-4.
3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

原厂资料：

Specificity / Sensitivity

Each antibody in the MYPT1 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. The MYPT1 Antibody is not likely to cross-react with MYPT2 nor does it cross-react with other family members. 

Source / Purification

Phospho-specific polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser507, Ser668, Thr853, and Thr696 of human MYPT1. MYPT1 Antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human MYPT1. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). 
 The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members also include MBS85, MYPT3 and TIMAP (4). 
 Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).

Phospho-MYPT1 (Ser507) and phospho-MYPT1 (Ser668) antibodies are directed at sites that were identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at these sites was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
2. Terrak, M. et al. (2004) Nature 429, 780-4.
3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies