TCTP (D10F2) Rabbit mAb

• 产品编号：CST-5128S      品牌：CST       原厂货号：5128S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TCTP (D10F2) Rabbit mAb能够检测内源性TCTP总蛋白。该单克隆抗体是由合成的人源的针对TCTP的羧基末端的肽段免疫动物生产的。翻译控制的肿瘤蛋白（TCTP/p23/HRF）是一种广泛表达的高度保守的蛋白质，参与各种细胞过程，如它在慢性过敏性疾病可作为组胺释放因子（1）。TCTP以细胞周期依赖性的方式结合微管蛋白，并与有丝分裂纺锤体相关联（2）。此外，TCTP与肌动蛋白细胞骨架相互作用来调节细胞的形状（3）。在有丝分裂中，TCTP可被PLK磷酸化Ser46，降低微管稳定性（4,5）。 TCTP与小GTP酶Rheb相互作用，可能作为一个GEF，从而激活TORC1途径和控制细胞的生长和增殖（6,7）。TCTP也被证明参与了细胞凋亡和细胞应激（8-11）。在培养的细胞中，TCTP表达减少可能会导致恶性表型的缺失（12）。

1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

原厂资料：

Specificity / Sensitivity

TCTP (D10F2) Rabbit mAb recognizes endogenous levels of total TCTP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCTP protein.

Background

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).

1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody