Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

• 产品编号：CST-5127S      品牌：CST       原厂货号：5127S
• 产品分类：抗体 > 二抗 > 酶标记
• 应用分类：

描述：

Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)识别天然的兔源IgG.不能与变性的和缺少重链(50KDa)和轻链(25KDa)的兔源IgG结合。单克隆抗体是通过采用天然的全兔IgG免疫动物生产的。Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)仅与天然IgG发生反应，不能与变性还原的兔源IgG的重链和轻链结合。当免疫沉淀后进行western blotting时，一抗结合的变性的兔源IgG轻链和重链的位置大约分别在25和50KDa，在随后的western blot中，会有相同分子量的条带模糊的蛋白。采用 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)了一减少这种问题的发生。

原厂资料：

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Background

Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.