RCC2 (D14F3) Rabbit mAb

• 产品编号：CST-5104S      品牌：CST       原厂货号：5104S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RCC2 (D14F3) Rabbit mAb兔单抗检测内源性RCC2总蛋白。通过人工合成人源RCC2蛋白羧基端相应的多肽片段去免疫动物从而制备出单克隆抗体。RCC2/TD-60蛋白是鸟嘌呤核苷交换因子（guanine nucleotide exchange factors）的RCC1 (regulator of chromosome condensation 1)家族成员。RCC2/TD-60蛋白与染色体乘客复合体(chromosomal passenger complex，CPC)有关联，CPC也由aurora B kinase, borealin, INCENP (inner centromere protein)和survivin组成。CPC在有丝分裂的不同阶段起着作用，它能与微管相互作用并且对适当的染色质分离和胞质分裂是需要的。aurora B kinase的调节在CPC的调节中是起着关键作用(1,2)。在有丝分裂后期，RCC2/TD-60对纺锤体组装和survivin与aurora B的招募是需要的(3)。RCC2/TD-60蛋白对体外aurora B激活和CPC定位到着丝粒也是需要的(4)。

1. Vader, G. et al. (2006) J Cell Biol 173, 833-7.
2. Ruchaud, S. et al. (2007) Nat Rev Mol Cell Biol 8, 798-812.
3. Mollinari, C. et al. (2003) Dev Cell 5, 295-307.
4. Rosasco-Nitcher, S.E. et al. (2008) Science 319, 469-72.

原厂资料：

Specificity / Sensitivity

RCC2 (D14F3) Rabbit mAb detects endogenous levels of total RCC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCC2 protein.

Background

RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC (reviewed in 1,2). In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B (3). RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres (4).

1. Vader, G. et al. (2006) J Cell Biol 173, 833-7.
2. Ruchaud, S. et al. (2007) Nat Rev Mol Cell Biol 8, 798-812.
3. Mollinari, C. et al. (2003) Dev Cell 5, 295-307.
4. Rosasco-Nitcher, S.E. et al. (2008) Science 319, 469-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.