ASCT2 (G11) Antibody

• 产品编号：CST-5100S      品牌：CST       原厂货号：5100S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ASCT2 (G11) Antibody用于检测内源性ASCT2总蛋白水平多克隆抗体是通过合成对应于人ASCT2蛋白氨基端序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。钠离子依赖型中性氨基酸转运蛋白2(ASCT2或SLC1A5)是一种中性氨基酸转运蛋白，它主要调节必需氨基酸与SLC7A5双向转运蛋白的连接(1,2)。在肝细胞中，ASCT2可能是主要的谷氨酸转运蛋白，并且被认为可以为肿瘤细胞的生长提供必需氨基酸(3)。另外一些证据表明，ASCT2在mTORC1信号通路中发挥某些作用，并且它对于抑制自噬也是必需的(4,5)。细胞表面的ASCT2是一些与传染性免疫缺陷病相关的哺乳类干扰逆转录病毒的受体；胞外一个小区域(ECL2)的变化都会导致物种特异性的受体功能的改变(6)。

1. Utsunomiya-Tate, N. et al. (1996) J Biol Chem 271, 14883-90.
2. Bröer, S. (2008) Physiol Rev 88, 249-86.
3. Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G1062-73.
4. Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol 293, C55-63.
5. Nicklin, P. et al. (2009) Cell 136, 521-34.
6. Marin, M. et al. (2003) J Virol 77, 2936-45.

原厂资料：

Specificity / Sensitivity

ASCT2 (G11) Antibody detects endogenous levels of total ASCT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ASCT2 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6).

1. Utsunomiya-Tate, N. et al. (1996) J Biol Chem 271, 14883-90.
2. Bröer, S. (2008) Physiol Rev 88, 249-86.
3. Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G1062-73.
4. Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol 293, C55-63.
5. Nicklin, P. et al. (2009) Cell 136, 521-34.
6. Marin, M. et al. (2003) J Virol 77, 2936-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.