Apaf-1 (R205) Antibody

• 产品编号：CST-5088S      品牌：CST       原厂货号：5088S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Apaf-1 (R205) Antibody 能够检测内源的Apaf-1 总蛋白。该多克隆抗体是采用合成的人源Apaf-1蛋白Arg205位点周围残基相对应的多肽来免疫动物而获得。抗体是通过protein A 和多肽亲和层析法纯化。凋亡蛋白酶激活因子 1 (Apaf-1)是凋亡过程中激活caspase-9的一个重要信号蛋白，人们最初把它鉴定为哺乳动物中与C. elegans 凋亡调节蛋白CED-4同源的蛋白(1)。在细胞色素C与dATP存在下，凋亡因子Apaf-1与caspase-9形成复合体，极大的促进了caspase-9的激活，进而导致caspase-3的激活 (2,3)。此蛋白包含一个N末端的CARD域，一个中间的CED-4同源域和C末端的多个WD-40重复。通过在CARD结构域之后的小片段插入一个多余的WD-40重复 (4)等可变剪切，Apaf-1能够表达多种亚型。Apaf-1敲除小鼠模型的研究表明，其存在普遍的凋亡缺陷并且能够抵抗很多凋亡刺激(5,6)。

1. Zou, H. et al. (1997) Cell 90, 405-13.
2. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
3. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
4. Benedict, M.A. et al. (2000) J Biol Chem 275, 8461-8.
5. Cecconi, F. et al. (1998) Cell 94, 727-37.
6. Yoshida, H. et al. (1998) Cell 94, 739-50.

原厂资料：

Specificity / Sensitivity

Apaf-1 (R205) Antibody detects endogenous levels of total Apaf-1 protein.

Source / Purification

Polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg205 of human Apaf-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).

1. Zou, H. et al. (1997) Cell 90, 405-13.
2. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
3. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
4. Benedict, M.A. et al. (2000) J Biol Chem 275, 8461-8.
5. Cecconi, F. et al. (1998) Cell 94, 727-37.
6. Yoshida, H. et al. (1998) Cell 94, 739-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody