Argonaute 3 (D15D2) Rabbit mAb

• 产品编号：CST-5054S      品牌：CST       原厂货号：5054S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Argonaute 3 (D15D2) Rabbit mAb兔单抗检测内源性的Argonaute 3总蛋白水平。该单克隆抗体是经由合成的对应于人Argonaute 3蛋白Val160位点周围序列的肽段免疫动物而生产的。高等真核细胞中，Small non-coding RNAs是重要的基因表达调节因子(1,2)。多种类别的small RNAs都已经被鉴定，例如包含short interfering RNAs (siRNAs)(3)、microRNAs (miRNAs)(4)和Piwi-interacting RNAs (piRNAs)(5)。MicroRNAs长度大约21核苷酸，其涉及到多种细胞内过程包括细胞生长、分化和应激反应(1,2)。MicroRNAs通过介导mRNA翻译或稳定来调节基因表达(2)。MicroRNAs的功能发挥是与蛋白组分构成micro-ribonucleoproteins (miRNPs)复合物(2)。在这些复合物中有一类重要的成分是Argonaute蛋白(1,2)。哺乳类Argonaute家族有四个成员，仅Argonaute 2(Ago2)拥有切片内切酶活性(1,2)。Argonaute蛋白参与microRNA介导基因沉默的不同的阶段，例如翻译的抑制和mRNA转化(1)。

1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23.
2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14.
3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7.
4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8.
5. Girard, A. et al. (2006) Nature 442, 199-202.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Argonaute 3 (D15D2) Rabbit mAb recognizes endogenous levels of total Argonaute 3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val160 of human Argonaute 3 protein.

Background

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).

1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23.
2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14.
3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7.
4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8.
5. Girard, A. et al. (2006) Nature 442, 199-202.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.