mTOR (7C10) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-5043S      品牌：CST       原厂货号：5043S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

mTOR (7C10) Rabbit mAb (Alexa Fluor ® 488 Conjugate)兔单抗可以检测内源性mTOR总蛋白。该单克隆抗体是采用合成的与人mTOR蛋白Ser2481周围序列相对应的肽段免疫动物而生产的。此抗体在最佳条件下结合了Alexa Fluor ® 488（ F/P 为2-6）。CST公司的此抗体结合了Alexa Fluor® 488 荧光染料，已经过内部验证，可以直接用于人类细胞的流式细胞术。此抗体与未标记的mTOR (7C10) Rabbit mAb 兔单抗#2983具有同样的物种交叉反应。哺乳动物雷帕霉素（rapamycin）靶蛋白（mTOR，FRAP，RAFT）是一种丝氨酸/苏氨酸蛋白激酶（1-3），其作为ATP和氨基酸的传感器，可以有效调控营养供应及细胞的生长（4,5）。当营养充分时，mTOR响应磷脂酸介导的信号，进而正向调节p70 S6 激酶，并参与失活eIF4E的抑制剂4E-BP1。这些信号导致特定的mRNA亚群的翻译。mTOR可以被Akt信号通路的PI3K激酶磷酸化修饰Ser2448位点，也能自我磷酸化Ser2481位点（7,8）。mTOR的在细胞生长和动态平衡中起着关键作用，在肿瘤中可能存在调控失常。因此，目前很多研究将mTOR作为一个潜在的抗肿瘤治疗靶点（9）。

1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
2. Brown, E.J. et al. (1994) Nature 369, 756-8.
3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
6. Fang, Y. et al. (2001) Science 294, 1942-5.
7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Horse

Specificity / Sensitivity

mTOR (7C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total mTOR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated mTOR (7C10) Rabbit mAb #2983.

Background

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
2. Brown, E.J. et al. (1994) Nature 369, 756-8.
3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
6. Fang, Y. et al. (2001) Science 294, 1942-5.
7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.