ERp72 (D70D12) XP? Rabbit mAb

• 产品编号：CST-5033P      品牌：Cell Signaling Technology       原厂货号：5033P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ERp72 (D70D12) XP® Rabbit mAb兔单抗识别内源性的ERp72总蛋白。该单克隆抗体用与人类ERp72蛋白中Met279位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。在分泌蛋白合成过程中，其转位到内质网（ER）中进行翻译后修饰和正确折叠。为了形成其天然构象，很多分泌蛋白需要形成分子间或分子内二硫键（1）。这个过程称为氧化蛋白折叠。蛋白二硫键异构酶（PDI）催化这些二硫键的形成和异构，其有2个硫氧还蛋白同源物（2）。其它拥有硫氧还蛋白同源结构域的内质网常驻蛋白，包括ER应激蛋白72（ERp72），组成了PDI家族（3,4）。ERp72包含三个硫氧还蛋白同源结构域（3），在二硫键的形成和异构中扮演重要作用（3,4）。

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Mazzarella, R.A. et al. (1990) J Biol Chem 265, 1094-101.
4. Satoh, M. et al. (2005) Cell Stress Chaperones 10, 278-84.

原厂资料：

Specificity / Sensitivity

ERp72 (D70D12) XP® Rabbit mAb detects endogenous levels of total ERp72 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Met279 of human ERp72 protein.

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) during synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess thioredoxin homology domains, including ER stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds (3,4).

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Mazzarella, R.A. et al. (1990) J Biol Chem 265, 1094-101.
4. Satoh, M. et al. (2005) Cell Stress Chaperones 10, 278-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.