Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb

• 产品编号：CST-5031S      品牌：CST       原厂货号：5031S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb只能检测内源的在Tyr980/981位点磷酸化的Jak3蛋白。在有些反应中，此抗体也能检测单个位点磷酸化的蛋白。此单克隆抗体是通过合成人源对应的Jak3 Tyr980/981位点周围的磷肽段来免疫动物而获得。Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活（1）。除了细胞因子受体的激活方式，Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷酸化酪氨酸结合区域。Jak3 主要是在造血细胞中表达并对免疫细胞的功能和发育有作用(6-8)。它能结合到共有的γ 亚单元 (γc) 并与一些细胞因子（包括IL-2, IL-4, IL-7, IL-9 和 IL-15）共用受体亚单元(9)。在缺失Jak3的条件下，IL-2信号通路和Stat5的激活是高度缺陷的(10,11)。Jak3能在多个位点磷酸化，包括在活性环内的Tyr980和981位点(12-14)。

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Thomis, D.C. et al. (1995) Science 270, 794-7.
7. Nosaka, T. et al. (1995) Science 270, 800-2.
8. Park, S.Y. et al. (1995) Immunity 3, 771-82.
9. Russell, S.M. et al. (1994) Science 266, 1042-5.
10. Johnston, J.A. et al. (1994) Nature 370, 151-3.
11. Oakes, S.A. et al. (1996) Immunity 5, 605-15.
12. Zhou, Y.J. et al. (1997) Proc Natl Acad Sci USA 94, 13850-5.
13. Cheng, H. et al. (2008) Mol Cell Biol 28, 2271-82.
14. Rikova, K. et al. (2007) Cell 131, 1190-203.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb detects endogenous levels of Jak3 when phosphorylated at Tyr980/981. Some reactivity may exist with single phosphorylation at these sites.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Tyr980/981 of human and mouse Jak3 protein.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common γ subunit (γc) and a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation is highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within its activation loop (12-14).

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Thomis, D.C. et al. (1995) Science 270, 794-7.
7. Nosaka, T. et al. (1995) Science 270, 800-2.
8. Park, S.Y. et al. (1995) Immunity 3, 771-82.
9. Russell, S.M. et al. (1994) Science 266, 1042-5.
10. Johnston, J.A. et al. (1994) Nature 370, 151-3.
11. Oakes, S.A. et al. (1996) Immunity 5, 605-15.
12. Zhou, Y.J. et al. (1997) Proc Natl Acad Sci USA 94, 13850-5.
13. Cheng, H. et al. (2008) Mol Cell Biol 28, 2271-82.
14. Rikova, K. et al. (2007) Cell 131, 1190-203.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody