MLK1 (D66E2) Rabbit mAb

• 产品编号：CST-5029S      品牌：CST       原厂货号：5029S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MLK1 (D66E2) Rabbit mAb兔单抗能检测内源性总MLK1蛋白水平。该抗体也能与未知起源的45 kDa蛋白发生交叉反应该单克隆抗体通过使用与人源MLK1蛋白亮氨酸1070周围残基相一致的合成肽段免疫动物而获得。混合系激酶MLKs属于有丝分裂原活化的激酶的激酶的激酶(MAPKKK)双特异性蛋白激酶家族。该激酶在序列水平不是很保守，MLK1、2、3共有一个保守结构域，其他蛋白-蛋白结合区域包含一个催化核心两个异亮氨酸/亮氨酸拉链基序(1)。MLK1更倾向于刺激JNK(c-Jun 胺端激酶)途径，以应答激动剂和应激反应(2)。虽然各种磷酸化都需要MLK1的全激活，活化环内的两个自磷酸化位点（丝氨酸308位点和苏氨酸312位点）被认为是主要的活化残基(3)。神经管细胞中，MLK1能作用于下游小G蛋白Rac1、Cdc42和上游MKK4、MKK7，以促进凋亡的发生（2）。

1. Dorow, D.S. et al. (1993) Eur J Biochem 213, 701-10.
2. Xu, Z. et al. (2001) Mol Cell Biol 21, 4713-24.
3. Durkin, J.T. et al. (2004) Biochemistry 43, 16348-55.

原厂资料：

Specificity / Sensitivity

MLK1 (D66E2) Rabbit mAb detects endogenous levels of total MLK1 protein. The antibody also cross-reacts with a 45 kDa protein of unknown origin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1070 of human MLK1 protein.

Background

Mixed-lineage kinases (MLKs) belong to the mitogen activated kinase kinase kinase (MAPKKK) family of dual-specificity protein kinases. While not particularly well conserved at the sequence level, MLK1, 2 and 3 share a conserved domain structure consisting of a catalytic core and two isoleucine/leucine zipper motifs among other protein-protein binding domains (1). MLK1 preferentially stimulates the JNK (c-Jun amino-terminal kinase) pathway in response to agonists and stress (2). Although multiple phosphorylation events are required for full activation of MLK1, two autophosphorylation sites within the activation loop (Ser308 and Thr312) appear to be the predominant activation residues (3). In neuronal cells, MLK1 appears to function downstream of the small G-proteins Rac1 and Cdc42 and upstream of MKK4 and MKK7 to promote apoptosis (2).

1. Dorow, D.S. et al. (1993) Eur J Biochem 213, 701-10.
2. Xu, Z. et al. (2001) Mol Cell Biol 21, 4713-24.
3. Durkin, J.T. et al. (2004) Biochemistry 43, 16348-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody