Bax (D2E11) Rabbit mAb

• 产品编号：CST-5023S      品牌：CST       原厂货号：5023S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bax (D2E11) Rabbit mAb 兔单抗能够检测内源 Bax 总蛋白。该单克隆抗体是采用合成的人源 Bax Leu45位点周围残基相对应的肽段来免疫动物而获得。Bcl-2家族由一系列进化保守的蛋白组成，它们都包含Bcl-2同源结构域，能通过控制线粒体膜的通透性和释放细胞色素C来调节凋亡（1-3）。目前，研究已经证明有四种BH结构域 (BH1-4)能够介导蛋白的相互作用。根据功能和结构的同源性，此家族蛋白可分为三组：促进生存的成员，包括Bcl-2、Bcl-xL、Mcl-1、A1 和 Bcl-w；促进凋亡的蛋白，包括Bax、Bak 和 Bok；仅含有BH3结构域的蛋白Bad、Bik、Bid、Puma、Bim、Bmf、Noxa 和 Hrk。在Bcl-2家族成员间，促进死亡和抑制死亡成员间的相互作用构成了变阻模型，即通过促死亡蛋白和抑制死亡蛋白的比例来决定细胞的命运（4）。因此，促生存蛋白通过结合和对抗促凋亡成员来发挥作用。通常来讲，仅含有BH3结构域的蛋白能结合和对抗促生存蛋白来促进凋亡（5）。因此，在这个系统中可能存在一些冗余的调节，比如这些家族成员的组织特异性转录和转录后翻译的调节，其可能发挥不同的生理作用。Bax 是细胞内通过线粒体胁迫而诱导凋亡过程中一个重要的组分(6)。受到凋亡的刺激后，Bax 形成链式聚合物，并从细胞质转运到线粒体膜上(7)。通过与线粒体膜上的 pore 蛋白互作，Bax 增加了膜的通透性，这使得细胞色素C从线粒体释放，从而激活caspase-9并起始caspase 激活的凋亡信号通路 (8,9)。

1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
6. Wei, M.C. et al. (2001) Science 292, 727-30.
7. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci USA 95, 4997-5002.
8. Narita, M. et al. (1998) Proc Natl Acad Sci USA 95, 14681-6.
9. Marzo, I. et al. (1998) Science 281, 2027-31.
10. Brimmell, M. et al. (1998) Oncogene 16, 1803-12.

原厂资料：

Specificity / Sensitivity

Bax (D2E11) Rabbit mAb detects endogenous levels of total Bax protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu45 of human Bax protein.

Background

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.Bax is a key component for cellular induced apoptosis through mitochondrial stress (6). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (7). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (8,9).

1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
6. Wei, M.C. et al. (2001) Science 292, 727-30.
7. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci USA 95, 4997-5002.
8. Narita, M. et al. (1998) Proc Natl Acad Sci USA 95, 14681-6.
9. Marzo, I. et al. (1998) Science 281, 2027-31.
10. Brimmell, M. et al. (1998) Oncogene 16, 1803-12.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.