PABP1 Antibody

• 产品编号：CST-4992S      品牌：CST       原厂货号：4992S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PABP1 Antibody检测内源性PABP1和PABP3总蛋白水平。通过人工合成人源PABP1蛋白氨基末端附近相应序列的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Poly(A)-binding protein 1 (PABP1)与mRNA的3' poly(A)尾及eIF4F蛋白相联系(1,2)。 eIF4F是一个复合体，它的功能主要有mRNA 5'帽子结构 识别(eIF4E)、RNA解旋酶呈递到5'端区域(eIF4A)、mRNA与核糖体建立连接(eIF4G)、通过 eIF4G和poly(A) binding protein (PABP)相互作用使mRNA环化。而研究显示PABP1有多种功能，包括翻译起始、mRNA稳定和mRNA降解(3,4)。PABP的磷酸化能提高真核生物RNA结合，并且PABP1在细胞核与细胞质穿梭(5,6)。甲基化转移酶CARM1蛋白使PABP1蛋白在Arg455和Arg460位点发生甲基化(7,8)；然而这个甲基化后的功能还没有确定。

1. Sachs, A. B. et al. (1986) Cell 45, 827-835.
2. Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
3. Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
4. Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
5. Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
6. Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
7. Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
8. Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish, Bovine

Specificity / Sensitivity

PABP1 Antibody detects endogenous levels of total PABP1 and PABP3 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human PABP1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.

1. Sachs, A. B. et al. (1986) Cell 45, 827-835.
2. Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
3. Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
4. Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
5. Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
6. Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
7. Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
8. Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.