NALP1 Antibody

• 产品编号：CST-4990S      品牌：CST       原厂货号：4990S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NALP1抗体能够检测内源NALP1蛋白总体水平。此抗体也能检测70 kDa蛋白，据预测可能是与是缺少富含亮氨酸区域的短形式的NALP1(NALP1s)(7)。此多克隆抗体是通过合成人源对应的NALP1 Gly1081位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。NALP1 (DEFCAP/NAC/CARD7)是NLR (Nod样受体)家族成员，它被发现可能调控凋亡和炎症反应(1-5)。从结构上看, NALP 包含了N-末端的PYRIN域，紧随其后的是核酸结合位点(NBS), 富含亮氨酸重复的区域(LRR)和C-末端CARD域。NALP1与caspase-2的相互作用很强，但是与caspase-9相互作用很弱,当其过表达后能诱导凋亡(3)。与相关的Ced-4家族成员Apaf-1相似,它也参与了细胞色素c-依赖级联反应的激活(2)。它也是"炎性复合体"中的组成部分，另外还有caspase-1, caspase-5 和 Pycard/ASC, 此复合体在促炎症细胞因子样IL-1β的形成中发挥了重要的作用(6)。从NALP1鉴定到两个重要的同型体,一个是在LRR区的44个氨基酸区域不同(3)。另外，如NALP3, 一个缺乏LRR短形式的NALP1(NALP1s)很可能也存在(7)。NALP1基因的多态性与自身免疫性疾病(8)和对毒素易感(9)有关。

1. Bertin, J. and DiStefano, P.S. (2000) Cell Death Differ. 7, 1273-1274.
2. Chu, Z.L. et al. (2001) J. Biol. Chem. 276, 9239-9245.
3. Hlaing, T. et al. (2001) J. Biol. Chem. 276, 9230-9238.
4. Martinon, F. et al. (2001) Curr. Biol. 11, R118-R120.
5. Fritz, J.H. et al. (2006) Nat. Immunol. 7, 1250-1257.
6. Martinon, F. et al. (2002) Mol. Cell 10, 417-426.
7. Kummer, J.A. et al. (2007) J. Histochem. Cytochem. 55, 443-452.
8. Jin, Y. et al. (2007) N. Engl. J. Med. 356, 1216-1225.
9. Boyden, E.D. and Dietrich, W.F. (2006) Nat. Genet. 38, 240-244.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

NALP1 Antibody detects endogenous levels of total NALP1 protein. This antibody also detects a 70 kDa protein that correlates with a predicted short form (NALP1s) that lacks the leucine repeat region (7).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide that corresponds to a region surrounding Gly1081 of human NALP1 protein. Antibodies were purified by peptide affinity chromatography.

Background

NALP1 (DEFCAP/NAC/CARD7) is an NLR (Nod-like receptor) family member that has been implicated in the regulation of apoptosis and inflammatory responses (1-5). Structurally, NALP contains an amino-terminal PYRIN domain, followed by a nucleotide-binding site (NBS), a leucine rich repeat region (LRR), and a carboxy-terminal CARD domain. NALP1 and interacts strongly with caspase-2 and weakly with caspase-9, and induces apoptosis when overexpressed (3). Similar to a related Ced-4 family member Apaf-1, it was also shown to be involved in cytochrome c-dependent caspase activation (2). It has also been shown to be part of the "inflammasome" comprised of caspase-1, caspase-5, and Pycard/ASC, which is critical in the processing of pro-inflammatory cytokines like IL-1β (6). Two major isoforms were identified for NALP1, which differ in a 44 amino acid region within the LRR (3). In addition, like NALP3, a short NALP1 isoform lacking the LRR (NALP1s) likely exists (7). Polymorphisms in NALP1 have been associated with autoimmune disease (8) and susceptibility to toxins (9).

1. Bertin, J. and DiStefano, P.S. (2000) Cell Death Differ. 7, 1273-1274.
2. Chu, Z.L. et al. (2001) J. Biol. Chem. 276, 9239-9245.
3. Hlaing, T. et al. (2001) J. Biol. Chem. 276, 9230-9238.
4. Martinon, F. et al. (2001) Curr. Biol. 11, R118-R120.
5. Fritz, J.H. et al. (2006) Nat. Immunol. 7, 1250-1257.
6. Martinon, F. et al. (2002) Mol. Cell 10, 417-426.
7. Kummer, J.A. et al. (2007) J. Histochem. Cytochem. 55, 443-452.
8. Jin, Y. et al. (2007) N. Engl. J. Med. 356, 1216-1225.
9. Boyden, E.D. and Dietrich, W.F. (2006) Nat. Genet. 38, 240-244.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.