Catenin δ-1 Antibody

• 产品编号：CST-4989S      品牌：CST       原厂货号：4989S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Catenin δ-1抗体可以识别内源性总的Catenin δ-1蛋白。合成对应人Catenin δ-1蛋白的多肽免疫动物获得单克隆抗体。抗体经过蛋白A和肽亲和层析纯化。Catenin δ-1（p120 catenin）氨基末端有卷曲螺旋，带有多个磷酸化位点以及一个由42个氨基酸重复10次构成的Armadillo重复区域组成的调控区域。羧基末端未发现已知功能（1）。Catenin δ-1调控细胞表面E-钙黏着蛋白转化使E-钙黏着蛋白在细胞与细胞黏着过程中发挥作用（2）。Catenin δ-1在钙黏着蛋白介导的粘附作用中既发挥正向作用也发挥负向调控作用（3）。Catenin δ-1还能够调控肌动蛋白丝的活性，随后修饰RhoA, Rac和cdc42蛋白（1）。作为β-catenin的类似物，catenin δ-1能够转位到细胞核，尽管其在该位置的作用还不清楚。许多研究显示catenin δ-1在多种癌细胞中不规则地表达或缺失，这提示catenin δ-1可能作为一个抑癌因子发挥作用（4）。

1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.

原厂资料：

Specificity / Sensitivity

Catenin δ-1 Antibody detects endogenous levels of total catenin δ-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human catenin δ-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).

1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.