Phospho-p63 (Ser160/162) Antibody

• 产品编号：CST-4981S      品牌：CST       原厂货号：4981S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-p63 (Ser160/162) Antibody能够检测内源性丝氨酸（160/162位）磷酸化的p63蛋白。该多克隆抗体是由合成的人源的针对TAp63-alpha蛋白丝氨酸（160/162位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。p53肿瘤抑制蛋白在细胞DNA损伤和其他基因组异常的应答中起着重要的作用。 p53的激活可导致无论是细胞周期停滞和DNA修复或细胞凋亡（1）。除了p53，哺乳动物细胞中含有两个p53家族成员，p63和p73，其结构和功能都与p53蛋白类似（2）。虽然p63蛋白可诱导p53-应答基因与细胞凋亡，突变的p6 3很少引起肿瘤（2）。肺部和头颈部鳞状细胞癌中经常可以观察到p63基因的扩增（2,3）。 p63基因包含一个选择性的转录起始位点，该位点能够产生缺乏反式激活结构域的40 kDa的deltaNp63，且在羧基末端选择性剪接产生α，β和γ异构体（3,4）。 TAp63-alpha (全长)包含丝氨酸残基后跟脯氨酸（Ser-Pro结构域），该结构域预计为有丝分裂过程中能够被cdk磷酸化的潜在的底物。这些磷酸化位点为丝氨酸（160位）、丝氨酸（162位）、丝氨酸（395位）、丝氨酸（455位）。

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Waltermann, A. et al. (2003) Oncogene 22, 5686-93.
3. Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7.
4. Yang, A. et al. (1999) Nature 398, 714-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus

Specificity / Sensitivity

Phospho-p63 (Ser160/162) Antibody detects endogenous levels of p63 only when phosphorylated at serine 160/162.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser160/160 of human TAp63-alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).TAp63-alpha (full-length) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis. Among these are Ser160, Ser162, Ser395, and Ser455. 

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Waltermann, A. et al. (2003) Oncogene 22, 5686-93.
3. Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7.
4. Yang, A. et al. (1999) Nature 398, 714-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.