Csk (C74C1) Rabbit mAb

• 产品编号：CST-4980P      品牌：Cell Signaling Technology       原厂货号：4980P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Csk (C74C1) Rabbit mAb兔单抗能检测内源性总Csk蛋白水平。该抗体不与其他蛋白发生交叉反应。该单克隆抗体是通过使用与人源Csk蛋白撷氨酸（399位点）周围氨基酸残基相一致的合成肽段免疫动物而获得的。羧端Src激酶(Csk)在非受体酪氨酸激酶中广泛表达，经SFK羧端酪氨酸的磷酸化负向调节Src家族激酶(SFKs) (1,2)。分子内磷酸化的羧端酪氨酸结合到SFK的SH2区域，导致SFK的折叠和失活(3)。Csk催化的SFK酪氨酸磷酸化是高度专业、排外的。SFK羧端酪氨酸是Csk唯一已知的生理学底物(4)。Csk包含SH2、SH3和激酶结构域。有证据表明SH2、SH3结构域对SFK的调节十分重要，Csk能被聚集到胞膜处，此处SFKs处于非活化状态。这一过程由Csk结合蛋白（Cbp, 也被称为 PAG)调节，该蛋白紧密结合到Csk的SH2区域(5)。SFK被胞外刺激因子活化，导致Cbp酪氨酸的磷酸化作用，为Csk提供“停泊位点”。Csk的聚集对SFK作用的终止形成了一个反馈机制(6)。

1. Nada, S. et al. (1991) Nature 351, 69-72.
2. Nada, S. et al. (1993) Cell 73, 1125-1135.
3. Lee, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14707-14712.
4. Imamoto, A. and Soriano, P. (1993) Cell 73, 1117-1124.
5. Kawabuchi, M. et al. (2000) Nature 404, 999-1003.
6. Matsuoka, H. et al. (2004) J. Biol. Chem. 279, 5975-5983.

原厂资料：

Specificity / Sensitivity

Csk (C74C1) Rabbit mAb detects endogenous levels of total Csk protein. This antibody does not cross-react with other proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid residue Val399 of human Csk.

Background

Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFKs) by phosphorylation of the SFK carboxy-terminal tyrosine (1,2). Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK (3). This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk (4). 
 Csk consists of an SH2, an SH3, and a kinase domain. There is evidence that the SH2 and SH3 domains are essential for the regulation of SFK, and Csk can be recruited to the membrane where SFKs are in an active state. This process is mediated by a Csk-binding protein (Cbp, also called PAG), which binds tightly to the SH2 domain of Csk (5). Activation of SFK by extracellular stimuli leads to the tyrosine phosphorylation of Cbp, generating docking sites for Csk. The recruitment of Csk forms a feedback mechanism for termination of SFK function (6).

1. Nada, S. et al. (1991) Nature 351, 69-72.
2. Nada, S. et al. (1993) Cell 73, 1125-1135.
3. Lee, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14707-14712.
4. Imamoto, A. and Soriano, P. (1993) Cell 73, 1117-1124.
5. Kawabuchi, M. et al. (2000) Nature 404, 999-1003.
6. Matsuoka, H. et al. (2004) J. Biol. Chem. 279, 5975-5983.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than
0.02% sodium azide. Store at –20°C. Do not aliquot the
antibody.