β-Actin (13E5) Rabbit mAb

• 产品编号：CST-4970P      品牌：Cell Signaling Technology       原厂货号：4970P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

β-Actin (13E5) Rabbit mAb识别内源性的β-actin总蛋白。该抗体可能与γ-actin(cytoplasmic isoform)发生交叉反应。它不与α-skeletal、α-cardiac、α-vascular smooth或γ-enteric平滑肌亚型发生交叉反应。通过人工合成与人源β-actin蛋白邻近氨基末端的氨基酸残基序列一致的肽段，免疫动物从而制备单克隆抗体。Actin是一个广泛存在的真核细胞蛋白质，其实细胞骨架的重要组成成分。在哺乳类动物中至少有六种亚型。非肌肉组织β-和γ-actin也称作细胞质肌蛋白，它们在非肌肉组织中主要表达，目的是控制细胞结构和运动(1)。α-cardiac和α-skeletal 肌蛋白分别在心脏横纹肌和骨骼肌表达；两个平滑肌肌蛋白（α-和γ-actin）各自主要地发现在血管平滑肌和肠道平滑肌。这些肌蛋白亚型调节肌肉细胞的收缩潜能(1)。Actin主要存在作为一个纤维聚合物F-actin。在一些进程例如细胞分裂、内吞作用或应激中回应细胞骨架重组信号，cofilin促进分裂和F-actin的解聚，从而导致在单体球状形式G-actin的增加(2)。Arp2/3复合物使F-actin片段稳定，并且促进新的肌蛋白微丝形成(2)。有研究报道actin是在主要乳腺肿瘤中高度磷酸化(3)。在凋亡条件下肌蛋白的分裂已经在体外、心肌和骨骼肌中观察(4-6)。通过caspase-3剪切的Actin可能加速泛素/蛋白酶体依赖的肌肉蛋白水解(6)。

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Dog, Horse

Specificity / Sensitivity

β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues near the amino-terminus of human β-actin.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.