Endonuclease G Antibody

• 产品编号：CST-4969S      品牌：CST       原厂货号：4969S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Endonuclease G Antibody 能够检测内源性水平的核酸内切酶G总蛋白。该多克隆抗体是采用合成的人源核酸内切酶G蛋白Cys122周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。核酸内切酶G（EndoG）是核编码的线粒体核酸酶。据报道，其在凋亡、DNA重组和细胞增殖等过程发挥作用（1-5）。 EndoG表达为一个含有一个氨基末端线粒体靶向序列的前体蛋白，该序列在蛋白质进入线粒体时被切除（1）。在细胞凋亡过程中，EndoG从线粒体释放，进而转位到细胞核，并与其他核酸酶一起触发细胞凋亡过程相关的DNA片段化（3,6,7）。

1. Côté, J. and Ruiz-Carrillo, A. (1993) Science 261, 765-769.
2. Parrish, J. et al. (2001) Nature 412, 90-94.
3. Li, L.Y. et al. (2001) Nature 412, 95-99.
4. Zhang, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 15782-15787.
5. Huang, K.J. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 8995-9000.
6. Widlak, P. et al. (2001) J. Biol. Chem. 276, 48404-48409.
7. Arnoult, D. et al. (2003) EMBO J. 22, 4385-4399.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Endonuclease G Antibody detects endogenous levels of total Endonuclease G protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys122 of human Endonuclease G. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation (1-5). EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria (1). During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process (3,6,7).

1. Côté, J. and Ruiz-Carrillo, A. (1993) Science 261, 765-769.
2. Parrish, J. et al. (2001) Nature 412, 90-94.
3. Li, L.Y. et al. (2001) Nature 412, 95-99.
4. Zhang, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 15782-15787.
5. Huang, K.J. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 8995-9000.
6. Widlak, P. et al. (2001) J. Biol. Chem. 276, 48404-48409.
7. Arnoult, D. et al. (2003) EMBO J. 22, 4385-4399.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.