c-IAP1 Antibody

• 产品编号：CST-4952S      品牌：CST       原厂货号：4952S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

c-IAP1 Antibody 能够检测内源c-IAP1总蛋白。在生理水平下，此抗体不与家族的其它成员交叉反应。该多克隆抗体是采用合成的c-IAP1蛋白前两个BIR结构域之间某区域残基相应的肽段来免疫动物而获得。抗体通过protein A 和多肽亲和层析法纯化。凋亡抑制因子蛋白(IAP) 家族包含一个进化上保守的凋亡抑制分支，此分支蛋白包含70个氨基酸的BIR (杆状病毒抑制重复) 域(1,2)。在人类中此家族的蛋白包含c-IAP1、c-IAP2、XIAP、surviving、livin 和NAIP。在癌细胞系和原癌中有IAP 家族成员的过表达，尤其是survivin和livin, 表明了这些蛋白在癌症发展过程中的重要作用(3-5)。一般来讲，IAP蛋白发挥作用是通过直接抑制几个caspases的活性, 包括caspase-3、caspase-7和caspase-9 (5,6)。另外，IAP家族的成员与线粒体蛋白Smac的结合阻止了它们与caspase-9的互作, 因此允许caspase的加工及其激活 (2)。

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

原厂资料：

Specificity / Sensitivity

c-IAP1 Antibody detects endogenous levels of c-IAP1. At physiological levels, cross reactivity was not detected with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region between the first two BIR domains of c-IAP1. Antibodies are purified by protein A and affinity chromatography.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.