IRF-5 Antibody (Rodent Specific)

• 产品编号：CST-4950S      品牌：CST       原厂货号：4950S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRF-5 Antibody (Rodent Specific)能够检测内源IRF-5蛋白总体水平。多克隆抗体是通过合成人源对应的IRF-5 Ser172位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录，例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。IRF-5在淋巴组织和外周血淋巴细胞中表达，并在受到病毒感染后参与到I类干扰素基因的诱导中(3)。IRF-5信号的激活有TLR信号通路上成员的参与，包括TLR7 和 MyD88 (4,5)。IRF-5在遗传上的多样性与信号通路上的混乱有关，如IFN信号通路不正常的激活, 如系统性的红斑狼疮(6,7)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Barnes, B.J. et al. (2001) J. Biol. Chem. 276, 23382-23390.
4. Takaoka, A. et al. (2005) Nature 434, 243-249.
5. Schoenemeyer, A. et al. (2005) J. Biol. Chem. 280, 17005-17012.
6. Sigurdsson, S. et al. (2005) Am. J. Hum. Genet. 76, 528-537.
7. Graham, G. et al. (2006) Nat. Genet. 38, 550-555.

原厂资料：

Specificity / Sensitivity

IRF-5 Antibody (Rodent Specific) detects endogenous levels of total IRF-5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser172 of mouse IRF-5 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).RF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by components of the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7).

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Barnes, B.J. et al. (2001) J. Biol. Chem. 276, 23382-23390.
4. Takaoka, A. et al. (2005) Nature 434, 243-249.
5. Schoenemeyer, A. et al. (2005) J. Biol. Chem. 280, 17005-17012.
6. Sigurdsson, S. et al. (2005) Am. J. Hum. Genet. 76, 528-537.
7. Graham, G. et al. (2006) Nat. Genet. 38, 550-555.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.