AID (30F12) Rabbit mAb

• 产品编号：CST-4949S      品牌：CST       原厂货号：4949S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AID (30F12) Rabbit mAb 兔单抗能够检测内源水平AID蛋白总体水平。此单克隆抗体是通过合成人源对应的AID Leu165位点周围的肽段来免疫动物而获得。活化诱导胞嘧啶核苷脱氨酶(AID) 由于与RNA编辑酶APOBEC-1高度同源，所以被认为能够修饰RNA。此功能并未在体外的研究中得到证实，但是AID具有明显的胞嘧啶核苷脱氨酶活性,并且其活性可被锌离子的螯合作用所抑制 (1)。在一些生物体中，B 细胞免疫系统能够特异性的识别一些感染因子, 并且远远多于免疫球蛋白基因片段所能编码的种类。可能的机制如体细胞突变, 同型开关重组，基因多样性转化和对免疫系统的特异性。对小鼠模型和 AID缺失病人的研究将3个机制与AID相互联系起来(2)。AID蛋白在生发中心母细胞和生发中心起源的淋巴瘤(Burkitt 淋巴瘤)中能够检测到, 但在是在前生发中心B细胞或后生发中心肿瘤(B细胞慢性淋巴细胞白血病和多发性骨髓瘤)中检测不到 (3)。

1. Muramatsu, M. et al. (1999) J. Biol. Chem. 274, 18470-18476.
2. Reynaud, C.A. et al. (2003) Nat. Immunol. 7, 631-638.
3. Pasqualucci, L. et al. (2004) Blood 104, 3318-3325.

原厂资料：

Specificity / Sensitivity

AID (30F12) Rabbit mAb detects endogenous levels of total AID protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu165 of human AID.

Background

Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation (1). 
 The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function (2). AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma) (3).

1. Muramatsu, M. et al. (1999) J. Biol. Chem. 274, 18470-18476.
2. Reynaud, C.A. et al. (2003) Nat. Immunol. 7, 631-638.
3. Pasqualucci, L. et al. (2004) Blood 104, 3318-3325.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.