HR6A/HR6B Antibody

• 产品编号：CST-4944S      品牌：CST       原厂货号：4944S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HR6A/HR6B Antibody能够检测内源性HR6A和HR6B总蛋白。该多克隆抗体是由合成的人源的针对HR6A蛋白氨基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。泛素结合(UBC)酶HR6A 和HR6B是酿酒酵母Rad6基因在哺乳动物中的同源基因产物(1)。在啤酒酵母中，Rad6促进细胞周期进程和泛素化组蛋白H2B (2,3)。周期蛋白依赖性激酶介导的HR6A Ser120在体磷酸化被认为在参与细胞周期进程的协调和时序性泛素化过程中是很重要的(4)。DNA损伤应答中，HR6A能够与p53 和p14ARF相互作用，但是敲除后的小鼠缺少HR6A 或者 HR6B显示正常的DNA损伤反应(5,6)。HR6B敲除的雄鼠呈现精子发生缺陷，HR6A敲除的雌鼠不能生产有生育能力的后代(6)。

1. Koken, M. H. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 8865-8869.
2. Ellison, K. S. et al. (1991) J. Biol. Chem. 266, 24116-24120.
3. Robzyk, K. et al. (2000) Science 287, 501-504.
4. Sarcevic, B. et al. (2002) EMBO J. 21, 2009-2018.
5. Lyakhovich, A. and Shekhar, M.P. (2003) Mol. Cell. Biol. 23, 2463-2475.
6. Roest, H. P. et al. (2004) Mol. Cell. Biol. 24, 5485-5495.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, D. melanogaster, Xenopus, Zebrafish

Specificity / Sensitivity

HR6A/HR6B Antibody detects endogenous levels of total HR6A and HR6B proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human HR6A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The ubiquitin-conjugating (UBC) enzymes HR6A and HR6B are the mammalian orthologues of the Saccharomyces cerevisiae Rad6 gene products (1). In S. cerevisiae, Rad6 facilitates cell cycle progression and ubiquitinates histone H2B (2,3). In vivo phosphorylation of HR6A Ser120 by cyclin-dependent kinases is thought to be important for the coordination and timing of ubiquitination events involved in cell cycle progression (4). In response to DNA damage, HR6A is known to interact physically with p53 and p14ARF, but knockout mice lacking HR6A or HR6B exhibit normal DNA damage responses (5,6). HR6B knockout males exhibit defective spermatogenesis, while HR6A knockout females fail to produce viable offspring (6). 

1. Koken, M. H. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 8865-8869.
2. Ellison, K. S. et al. (1991) J. Biol. Chem. 266, 24116-24120.
3. Robzyk, K. et al. (2000) Science 287, 501-504.
4. Sarcevic, B. et al. (2002) EMBO J. 21, 2009-2018.
5. Lyakhovich, A. and Shekhar, M.P. (2003) Mol. Cell. Biol. 23, 2463-2475.
6. Roest, H. P. et al. (2004) Mol. Cell. Biol. 24, 5485-5495.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.