IRF-2 Antibody

• 产品编号：CST-4943S      品牌：CST       原厂货号：4943S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRF-2 Antibody能够检测内源IRF-2蛋白总体水平。此多克隆抗体是通过合成人源对应的IRF-2 Asp327位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录，例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。与IRF家族的其它成员结构上相似, IRF-2既作为转录的激活因子又作为抑制因子。IRF-2促进几个基因的转录，包括组蛋白H4, VCAM1细胞粘连蛋白和MHC反式激活蛋白CIITA。相反的, IRF-2竞争性的结合到IRF-1启动子上并通过此相关的转录因子抑制其活性(3)。它的活性可能被诱导型的蛋白水解所调控(4)。IRF-2也潜在的与致癌有关(5,6)和淋巴的发育也有关系(7,8)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Harada, H. et al. (1989) Cell 58, 729-739.
4. Palombella, V.J. and Maniatis, T. (1992) Mol. Cell Biol. 12, 3325-3336.
5. Harada, H. et al. (1993) Science 259, 971-974.
6. Nguyen, H. et al. (1995) Oncogene 11, 537-544.
7. Matsuyama, T. et al. (1993) Cell 75, 83-97.
8. Lohoff, M. et al. (2000) J. Exp. Med. 192, 325-336.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

IRF-2 Antibody detects endogenous levels of total IRF-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp327 of human IRF-2 protein. Antibodies were purified by affinity chromatography.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).Structurally similar to other IRF family members, IRF-2 acts as both a transcription activator and repressor. IRF-2 promotes transcription of several genes, including histone H4, the VCAM1 cell adhesion protein, and CIITA, a MHC transactivator protein. Conversely, IRF-2 competitively binds to promoter sites utilized by IRF-1, repressing activation by this related transcription factor (3). Its activity may be controlled by inducible proteolysis (4). IRF-2 has also been associated with increased oncogenic potential (5,6) and lymphocyte development (7,8).

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Harada, H. et al. (1989) Cell 58, 729-739.
4. Palombella, V.J. and Maniatis, T. (1992) Mol. Cell Biol. 12, 3325-3336.
5. Harada, H. et al. (1993) Science 259, 971-974.
6. Nguyen, H. et al. (1995) Oncogene 11, 537-544.
7. Matsuyama, T. et al. (1993) Cell 75, 83-97.
8. Lohoff, M. et al. (2000) J. Exp. Med. 192, 325-336.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.