Phospho-PBK/TOPK (Thr9) Antibody

• 产品编号：CST-4941S      品牌：CST       原厂货号：4941S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PBK/TOPK (Thr9) Antibody能够检测内源性苏氨酸（9位）磷酸化的PBK/TOPK 蛋白。该多克隆抗体是由合成的人源的针对PBK/TOPK 蛋白苏氨酸（9位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。PBK/TOPK是一种丝氨酸/苏氨酸激酶，在有丝分裂过程中被磷酸化和活化(1)。PBK/TOPK由激酶亚结构域和一个羧基末端PDZ-结合结构域，该结构域被认为能够和肿瘤抑制蛋白hDlg相互作用(1)。在高度增殖的恶性肿瘤细胞系中已观察到PBK/TOPK的表达增加，而在终端分化的HL-60白血病细胞中观察到PBK/TOPK的显著下调(2,3)。PMA-诱导的针对PBK/TOPK的激酶活性也被观察到，且cdc2/cyclinB被认为可以离体磷酸化PBK/TOPK，可能的位点是第9位苏氨酸(1)。PBK/TOPK的潜在底物包括p38 MAPK 和c-Myc (3,4)。

1. Gaudet, S. et al. (2000) Proc. Natl. Acad. Sci. U S A 97, 5167-5172.
2. Simons-Evelyn, M. et al. (2001) Blood Cells Mol. Dis. 27, 825-829.
3. Nandi, A. et al. (2004) Blood Cells Mol. Dis. 32, 240-245.
4. Abe, Y. et al. (2000) J. Biol. Chem. 276, 21525-21531.
5. Matsumoto, S. et al. (2004) Biochem Biophys Res Commun. 325, 997-1004.

原厂资料：

Specificity / Sensitivity

Phospho-PBK/TOPK (Thr9) Antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Thr9 of human PBK/TOPK. Antibodies are purified by protein A and affinity chromatography.

Background

PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4). 

1. Gaudet, S. et al. (2000) Proc. Natl. Acad. Sci. U S A 97, 5167-5172.
2. Simons-Evelyn, M. et al. (2001) Blood Cells Mol. Dis. 27, 825-829.
3. Nandi, A. et al. (2004) Blood Cells Mol. Dis. 32, 240-245.
4. Abe, Y. et al. (2000) J. Biol. Chem. 276, 21525-21531.
5. Matsumoto, S. et al. (2004) Biochem Biophys Res Commun. 325, 997-1004.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.