Claudin-1 Antibody

• 产品编号：CST-4933P      品牌：Cell Signaling Technology       原厂货号：4933P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Claudin-1抗体可以识别内源性claudin-1蛋白。基于序列相似性，该抗体与claudin-2有交叉反应。合成小鼠claudin-1蛋白羧基末端及其临近氨基酸残基序列的多肽免疫动物获得单克隆抗体。抗体由蛋白A和肽亲和层析纯化获得。紧密连接或闭锁小带，可以在上皮和内皮细胞间形成阻碍液体的连续结构。它们调节细胞旁渗透性，维持细胞极性，封闭顶端和底外侧细胞表面跨膜蛋白运动。紧密连接由claudin和occludin跨膜蛋白构成，claudin和occludin参与了细胞骨架结点（1,2）。Claudin家族由23个结合膜蛋白质组成，它们的表达在多种组织中不同，这可能决定了上皮屏障的强度和组成（2,3）。Claudin-1最初在角蛋白细胞和正常哺乳动物上皮细胞中表达（4），在乳腺癌组织和乳腺癌细胞系中缺失或表达减少（5,6）。

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
3. Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
4. Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63.
5. Krämer, F. et al. (2000) Hum Genet 107, 249-56.
6. Swisshelm, K. et al. (1999) Gene 226, 285-95.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Claudin-1 Antibody detects endogenous levels of total claudin-1 protein. Based on sequence similarity, the antibody may cross-react with claudin-2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse claudin-1. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
3. Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
4. Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63.
5. Krämer, F. et al. (2000) Hum Genet 107, 249-56.
6. Swisshelm, K. et al. (1999) Gene 226, 285-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.