Hic-5 Antibody

• 产品编号：CST-4914S      品牌：CST       原厂货号：4914S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Hic-5抗体可以识别内源性总的Hic-5/ARA55蛋白。合成对应人Hic-5蛋白104位丙氨酸及其临近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体由蛋白A和肽亲和层析纯化获得。Hic-5是一个LIM家族成员最初被认为是TGFbeta1和过氧化氢诱导的基因，与雄激素受体辅助刺激因子ARA55几乎相同（1-3）。Hic-5与paxillin在结构上相关联，两个蛋白都定位在黏着斑上作为接头分子连接细胞外基质信号调节细胞骨架和细胞内信号通路（4,5）。类似于paxillin，Hic-5有4个LD继续和4个LIM结构域。Hic-5的表达可以影响细胞生长和分化（6-8）。在成纤维细胞衰老过程中能观察到Hic-5表达增加，在成系的成纤维细胞中过表达Hic-5会抑制细胞生长（8）。与paxillin不同的是，Hic-5在过氧化氢等氧化剂作用下可以转位到细胞核（9）。Hic-5在黏着斑和细胞核间的氧化还原反应信号通路中往返作为一种转录共激活因子协助一些转录因子发挥作用，包括Sp1和PPARgamma（7,9,10）。Hic-5 60位酪氨酸被CAKbeta和Fyn磷酸化可以使其结合下游含有SH2结构域的蛋白而激活Hic-5信号通路（11）。

1. Ohba, M. et al. (1994) J. Cell Biol. 126, 1079-1088.
2. Shibanuma, M. et al. (1994) J. Biol. Chem. 269, 26767-26774.
3. Fujimoto, N. et al. (1999) J. Biol. Chem. 274, 8316-8321.
4. Matsuya, M. et al. (1998) J. Biol. Chem. 273, 1003-1014.
5. Nishiya, N. et al. (2001) Mol. Cell. Biol. 21, 5332-5345.
6. Hu, Y. et al. (1999) Proc. Natl. Acad. Sci. 96, 10218-10223.
7. Drori, S. et al. (2005) Genes Dev. 19, 362-375.
8. Shibanuma, M. et al. (1997) Mol. Cell. Biol. 17, 1224-1235.
9. Shibanuma, M. et al. (2003) Mol. Biol. Cell 14, 1158-1171.
10. Shibanuma, M. et al. (2004) J. Cell. Biochem. 91, 633-645.
11. Ishino, M. et al. (2000) FEBS Lett. 474, 179-183.

原厂资料：

Specificity / Sensitivity

Hic-5 Antibody detects endogenous levels of total Hic-5/ARA55 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala104 of human Hic-5. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hic-5 is a LIM domain family member orginally identified as a TGFbeta1 and hydrogen peroxide inducible gene, and is nearly identical to the androgen receptor co-activator ARA55 (1-3). Hic-5 is structurally related to paxillin, and both proteins are localized to focal adhesions and thought to serve as adaptor molecules, linking signals from the extracellular matrix to cytoskeletal regulation and intracelluar signaling (4,5). Like paxillin, Hic-5 contains four LD motifs and four LIM domains. Expression of Hic-5 can affect cell growth and differentiation (6-8). Increased expression of Hic-5 is observed during cellular senescence in fibroblasts, and ectopic expression in immortalized fibroblasts suppressed cell growth (8). Unlike paxillin, Hic-5 may translocate to the nucleus in response to oxidants like hydrogen peroxide (9). It has been proposed that Hic-5 serves to shuttle redox signaling from focal adhesions to the nucleus where it acts as a transcriptional co-activator for some transciption factors including, Sp1 and PPARgamma (7,9,10). Phosphorylation of Hic-5 at Tyr60 by CAKbeta and Fyn may activiate Hic-5 signaling by allowing binding to downstream SH2 domain containing proteins (11).

1. Ohba, M. et al. (1994) J. Cell Biol. 126, 1079-1088.
2. Shibanuma, M. et al. (1994) J. Biol. Chem. 269, 26767-26774.
3. Fujimoto, N. et al. (1999) J. Biol. Chem. 274, 8316-8321.
4. Matsuya, M. et al. (1998) J. Biol. Chem. 273, 1003-1014.
5. Nishiya, N. et al. (2001) Mol. Cell. Biol. 21, 5332-5345.
6. Hu, Y. et al. (1999) Proc. Natl. Acad. Sci. 96, 10218-10223.
7. Drori, S. et al. (2005) Genes Dev. 19, 362-375.
8. Shibanuma, M. et al. (1997) Mol. Cell. Biol. 17, 1224-1235.
9. Shibanuma, M. et al. (2003) Mol. Biol. Cell 14, 1158-1171.
10. Shibanuma, M. et al. (2004) J. Cell. Biochem. 91, 633-645.
11. Ishino, M. et al. (2000) FEBS Lett. 474, 179-183.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody