Phospho-cdc25C (Ser216) (63F9) Rabbit mAb

• 产品编号：CST-4901S      品牌：CST       原厂货号：4901S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb 能够检测内源性丝氨酸（216位点）磷酸化的cdc25C蛋白。该单克隆抗体是有合成的人源的针对丝氨酸（216位点）的磷酸化肽段免疫动物生产的。cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

原厂资料：

Specificity / Sensitivity

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb detects endogenous levels of cdc25C only when phosphorylated at Ser216.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. 

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). 

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.