Rab6 Antibody

• 产品编号：CST-4879S      品牌：CST       原厂货号：4879S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab6 Antibody识别内源性的Rab6总蛋白水平。该多克隆抗体用与人类Rab6中Lys133位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。Rab6是小Rab GTP酶Ras超家族的成员，与内吞作用有关（1）。Rab6亚家族的三个成员（Rab6A、Rab6A'和Rab6B）结构相似，但却显示出不相重合的功能（2,3）。Rab6定位在高尔基体（4），调节膜结合的目标蛋白从高尔基体到内质网的反向转运（5-7），或者蛋白在胞吐时从高尔基体到内体的转运（8）。Rab6与微管马达蛋白如rabkinesin-6 (KIF20A)和dynein/dynactin复合体相互作用；Rab6相关的转运需要功能完整的微管系统（9,10）。Rab6也通过与Rab6效应蛋白相互作用帮助调节细胞运动和细胞周期检查点，包括dynein/dynactin蛋白DCTN1和GTP酶活性蛋白RABGAP1（11,12）。

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Echard, A. et al. (2000) Mol Biol Cell 11, 3819-33.
3. Del Nery, E. et al. (2006) Traffic 7, 394-407.
4. Goud, B. et al. (1994) Ann N Y Acad Sci 733, 340-3.
5. White, J. et al. (1999) J Cell Biol 147, 743-60.
6. Opdam, F.J. et al. (2000) J Cell Sci 113 ( Pt 15), 2725-35.
7. Martinez, O. et al. (1997) Proc Natl Acad Sci USA 94, 1828-33.
8. Grigoriev, I. et al. (2007) Dev Cell 13, 305-14.
9. Echard, A. et al. (1998) Science 279, 580-5.
10. Young, J. et al. (2005) Mol Biol Cell 16, 162-77.
11. Hill, E. et al. (2000) EMBO J 19, 5711-9.
12. Miserey-Lenkei, S. et al. (2006) EMBO J 25, 278-89.

原厂资料：

Specificity / Sensitivity

Rab6 Antibody detects endogenous levels of total Rab6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys133 of human Rab6 protein. Antibodies are purified by peptide affinity chromatography.

Background

Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A' and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6 related transport requires a functionally intact, microtubule system (9,10). Rab6 proteins help regulate cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12).

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Echard, A. et al. (2000) Mol Biol Cell 11, 3819-33.
3. Del Nery, E. et al. (2006) Traffic 7, 394-407.
4. Goud, B. et al. (1994) Ann N Y Acad Sci 733, 340-3.
5. White, J. et al. (1999) J Cell Biol 147, 743-60.
6. Opdam, F.J. et al. (2000) J Cell Sci 113 ( Pt 15), 2725-35.
7. Martinez, O. et al. (1997) Proc Natl Acad Sci USA 94, 1828-33.
8. Grigoriev, I. et al. (2007) Dev Cell 13, 305-14.
9. Echard, A. et al. (1998) Science 279, 580-5.
10. Young, J. et al. (2005) Mol Biol Cell 16, 162-77.
11. Hill, E. et al. (2000) EMBO J 19, 5711-9.
12. Miserey-Lenkei, S. et al. (2006) EMBO J 25, 278-89.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody