HSP90 (C45G5) Rabbit mAb

• 产品编号：CST-4877P      品牌：Cell Signaling Technology       原厂货号：4877P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSP40 (C64B4) Rabbit mAb兔单抗识别内源性的HSP90总蛋白。此抗体与其它HSP蛋白没有交叉反应。该单克隆抗体用与人类HSP90中Asn300位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。HSP70和HSP90是正常情况下组成性表达的分子伴侣，能够保持蛋白合成稳态，并且在环境压力下被诱导（1）。HSP70和HSP90能够与未折叠蛋白相互作用，防止其不可逆的堆积，并且在ATP和共伴侣依赖的形式下催化其重新折叠（1）。HSP70的底物广泛，包括新合成和变性的蛋白，HSP90倾向于有更局限的底物，大部分是信号分子。HSP70和HSP90经常在多分子伴侣系统中协同发挥功能，它们至少需要一些共伴侣：HSP40、Hop和p23（2,3）。共伴侣调节分子伴侣的内在ATP酶活性或者招募分子伴侣到特定的底物或亚细胞区域中去（1,4）。当泛素连接酶CHIP与HSP70/HSP90复合物结合作为协同因子，未折叠的底物将被蛋白酶降解（4）。HSP70/HSP90的生物学功能应该不仅是其分子伴侣活性。它们也是核激素以及其它信号分子的成熟和激活所至关重要的（1,3）。它们在囊泡形成和蛋白转运中也扮演作用（2）。

1. Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816.
2. Young, J.C. et al. (2003) Trends Biochem. Sci. 28, 541-547.
3. Pratt, W.B. and Toft, D.O. (2003) Exp. Biol. Med. 228, 111-133.
4. Hohfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

HSP90 (C45G5) Rabbit mAb detects endogenous levels of total HSP90 protein. This antibody does not cross-react with other HSPs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asn300 of human HSP90.

Background

HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP and co-chaperone dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2).

1. Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816.
2. Young, J.C. et al. (2003) Trends Biochem. Sci. 28, 541-547.
3. Pratt, W.B. and Toft, D.O. (2003) Exp. Biol. Med. 228, 111-133.
4. Hohfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.