PTP-PEST (AG10) Mouse mAb

• 产品编号：CST-4864S      品牌：CST       原厂货号：4864S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PTP-PEST (AG10)鼠单抗可以检测内源性总的PTP-PEST 蛋白。该抗体与其他酪氨酸磷酸酯酶没有交叉反应。用人PTP-PEST重组蛋白免疫动物获得单克隆抗体。抗体可以识别氨基末端305个氨基酸残基中的一个抗原表位。PTP-PEST是一个广泛表达的胞浆蛋白酪氨酸磷酸酶，有多个富含脯氨酸的区域作为PTP-PEST结合配体或底物的结合位点（1）。PTP-PEST通过结合以及去磷酸化p130 Cas，paxillin，PSTPIP1，WASp和其他粘附分子以调控成纤维细胞粘附，迁移和胞质分裂（1-5）。通过对Shc, Pyk2, Fak 和WASp磷酸化进行调整，PTP-PEST可以降低淋巴细胞激活水平（1,6）。在哺乳动物上皮细胞中，EGF通过PTP-PEST协助去磷酸化Jak2，继而干扰催乳激素PRL信号通路（7）。PTP-PEST也能去磷酸化c-Ab1，c-Ab1可以影响PTP-PEST底物的磷酸化状态，这些底物包括paxillin, p130 Cas, Crk 和 PSTPIP1 (8)。

1. Davidson, D. and Veillette, A. (2001) EMBO J 20, 3414-26.
2. Garton, A.J. and Tonks, N.K. (1999) J Biol Chem 274, 3811-8.
3. Shen, Y. et al. (2000) J Biol Chem 275, 1405-13.
4. Angers-Loustau, A. et al. (1999) J Cell Biol 144, 1019-31.
5. Côté, J.F. et al. (2002) J Biol Chem 277, 2973-86.
6. Badour, K. et al. (2004) J Exp Med 199, 99-112.
7. Horsch, K. et al. (2001) Mol Endocrinol 15, 2182-96.
8. Cong, F. et al. (2000) Mol Cell 6, 1413-23.
9. Espejo, R. et al. (2010) Am J Physiol Cell Physiol 299, C454-63.
10. Souza, C.M. et al. (2012) J Biol Chem 287, 43180-90.

原厂资料：

Specificity / Sensitivity

PTP-PEST (AG10) Mouse mAb detects endogenous levels of total PTP-PEST protein. This antibody does not cross-react with other protein tyrosine pho sphatases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human PTP-PEST recombinant protein. The antibody recognizes an epitope within the amino-terminal 305 residues.

Background

PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASp, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak and WASp, PTP-PEST negatively regulates of lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substates such as paxillin, p130 Cas, Crk and PSTPIP1 (8).

1. Davidson, D. and Veillette, A. (2001) EMBO J 20, 3414-26.
2. Garton, A.J. and Tonks, N.K. (1999) J Biol Chem 274, 3811-8.
3. Shen, Y. et al. (2000) J Biol Chem 275, 1405-13.
4. Angers-Loustau, A. et al. (1999) J Cell Biol 144, 1019-31.
5. Côté, J.F. et al. (2002) J Biol Chem 277, 2973-86.
6. Badour, K. et al. (2004) J Exp Med 199, 99-112.
7. Horsch, K. et al. (2001) Mol Endocrinol 15, 2182-96.
8. Cong, F. et al. (2000) Mol Cell 6, 1413-23.
9. Espejo, R. et al. (2010) Am J Physiol Cell Physiol 299, C454-63.
10. Souza, C.M. et al. (2012) J Biol Chem 287, 43180-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody