RANK Antibody

• 产品编号：CST-4845S      品牌：CST       原厂货号：4845S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RANK 抗体能够检测内源RANK蛋白。此多克隆抗体是通过合成对应的RANK C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。RANK (NF-κB受体的激活剂)是肿瘤坏死因子(TNF)受体亚家族的一员，能够被其配体RANK(TRANCE/OPGL/ODF)激活,促进树突状细胞的存活和破骨细胞的分化(1-4)。尽管RANK的表达非常广泛，但是其细胞表面的表达确非常局限，仅局限于树突细胞和包皮成纤维细胞(1)。RANK包含一个383-氨基酸的胞内区域并与TRAF家族的特殊成员结合激活NF-κB和JNK(1,5)。RANKL/RANK信号通路也能通过Akt信号通路和上调存活蛋白包括Bcl-xL而导致存活信号通路(2,6)。RANK信号通路是潜在的治疗骨丢失和关节炎的靶标(7,8)。

1. Anderson, D.M. et al. (1997) Nature 390, 175-9.
2. Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
3. Lacey, D.L. et al. (1998) Cell 93, 165-76.
4. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
5. Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
6. Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
7. Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
8. Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.

原厂资料：

Specificity / Sensitivity

RANK Antibody detects endogenous levels of RANK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of RANK. Antibodies were purified by protein A and peptide affinity chromatography.

Background

RANK (receptor activator of NF-κB) is a member of the tumor necrosis factor (TNF) receptor subfamily that is activated by its ligand, RANKL (TRANCE/OPGL/ODF), to promote survival of dendritic cells and differentiation of osteoclasts (1-4). Although RANK is widely expressed, its cell surface expression may be more restricted to dendritic cells and foreskin fibroblasts (1). RANK contains a 383-amino acid intracellular domain that associates with specific members of the TRAF family to NF-κB and JNK activiation (1,5). RANKL/RANK signaling may also lead to survival signaling through activation of the Akt pathway and an upregulation of survival proteins, including Bcl-xL (2,6). RANK signaling has been implicated as a potential therapeutic to inhibit bone loss and arthritis (7,8).

1. Anderson, D.M. et al. (1997) Nature 390, 175-9.
2. Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
3. Lacey, D.L. et al. (1998) Cell 93, 165-76.
4. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
5. Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
6. Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
7. Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
8. Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.