Cox2 Antibody

• 产品编号：CST-4842S      品牌：CST       原厂货号：4842S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cox2抗体能够检测内源Cox2蛋白总体水平。此多克隆抗体是通过合成人源对应的Cox2序列的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。环氧合酶1(Cox1) 和环氧合酶2 (Cox2)与人源的同系物有60%的相似度,能够促进前列腺产生花生四烯酸(1,2)。 在大多数的组织中Cox1是组成型表达, Cox2 的表达要通过脂多糖(LPS) 和肽聚糖 (PGN)的诱导(3)。 PGN 激活 Ras, 导致了Raf 在 Ser338位点 和 Erk1/2 在 Tyr204位点的磷酸化。MAPK激酶信号通路的激活引起随后的IKKα/β的激活, IκBα在 Ser32/36位点的磷酸化, 和 NF-κB 的激活。 最终, 转录调控因子NF-κB的激活诱导了 Cox2基因的表达(4). LPS 和 PGN 的诱导在临床上表现为关节炎和细菌感染，如炎症引发的发热和败血病性休克 (5), 这些使得 Cox2 是抗炎药的一个重要靶标(3)。 Cox1 和 Cox2同时也在神经疾病如阿尔茨海默疾病中通过加强 γ-secretase 活性和β淀粉样蛋白产生过程中起了重要的作用(6).

1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Cox2 Antibody detects endogenous levels of total Cox2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Cox2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5), making Cox2 a useful target for therapeutic anti-inflammatory drugs (3). Cox1 and Cox2 also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).

1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.