Phospho-MARK Family (Activation Loop) Antibody

• 产品编号：CST-4836S      品牌：CST       原厂货号：4836S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MARK Family (Activation Loop) 抗体识别内源性的磷酸化MARK家族成员，MARK1蛋白threonine 215位点、MARK2蛋白threonine 208位点和MARK3蛋白threonine 234位点(A.K.A. 211 in isoforms 3-6)。该抗体不与MARK4反应。通过合成与人源MARK1蛋白threonine 215邻近氨基酸序列一致的肽段，免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。微管相关蛋白调节微管的稳定性和控制相关进程例如细胞极化/分化、轴突生长、细胞分裂和细胞器的运输(1)。丝氨酸/苏氨酸激酶的MARK (MAP/microtubule affinity-regulating kinases)家族(MARK1-4)被鉴定基于它们的有能力去磷酸化microtubule-associated proteins (MAPs)包括tau、MAP2和MAP4蛋白(2-6)。MARK蛋白在它们的微管结合区域里磷酸化MAPs，这就引起MAPs蛋白从微管上分离，并且增加了微管的动力(2-4)。至于tau蛋白，在阿尔茨海默病人中观察其磷酸化已经被假设有助于神经纤维缠结的形成。MARK激酶的过表达导致MAPs的过磷酸化 、形态学改变和细胞死亡(4)。肿瘤抑制激酶LKB1磷酸化MARK激酶和紧密相关的含有T-loops的AMP-kinases ，导致活性的增加(7)。

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.

原厂资料：

Specificity / Sensitivity

Phospho-MARK Family (Activation Loop) Antibody detects endogenous levels of phosphorylated MARK family members, MARK1 at threonine 215, MARK2 at threonine 208, and MARK3 at threonine 234 (A.K.A. 211 in isoforms 3-6) . This antibody does not react with MARK4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.