PHD-2/Egln1 (D31E11) Rabbit mAb

• 产品编号：CST-4835S      品牌：CST       原厂货号：4835S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PHD-2/Egln1 (D31E11)Rabbit mAb兔单抗识别内源性的总PHD-2/Egln1 蛋白。单克隆抗体由合成肽段免疫动物产生，该合成肽段与邻近人PHD-2/Egln1蛋Val226氨基酸残基序列一致。PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3)是Egln家族脯氨酸氢氧化酶成员。作为氧的感应器，他们对HIF的564位和402位脯氨酸羟基化，通过VHL/泛素化途径起始HIF降解的第一步（1,2）。PHD1高表达在一系列组织中，但PHD2 和 PHD3主要表达在心脏和骨骼肌（1,3）。在低氧条件下，HIF通过缺氧反应元件上调（PHD的mRNA水平4-7）。这三种酶也显示针对不同肽段的特异靶标蛋白，PHD1和PHD2既能能羟基化564位脯氨酸又能羟基化402位脯氨酸，但是PHD3仅能羟基化564位脯氨酸（2,8）。除了HIF外，PHD酶也显示催化RNA集聚合酶亚单元和肌形成蛋白的羟基化（3,9）。

1. Freeman, R.S. et al. (2003) Mol Cells 16, 1-12.
2. Villar, D. et al. (2007) Biochem J 408, 231-40.
3. Fu, J. et al. (2007) J Biol Chem 282, 12410-8.
4. D'Angelo, G. et al. (2003) J Biol Chem 278, 38183-7.
5. del Peso, L. et al. (2003) J Biol Chem 278, 48690-5.
6. Pescador, N. et al. (2005) Biochem J 390, 189-97.
7. Metzen, E. et al. (2005) Biochem J 387, 711-7.
8. Hirsilä, M. et al. (2003) J Biol Chem 278, 30772-80.
9. Mikhaylova, O. et al. (2008) Mol Cell Biol 28, 2701-17.

原厂资料：

Specificity / Sensitivity

PHD-2/Egln1 (D31E11) Rabbit mAb detects endogenous levels of total PHD-2/Egln1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val226 of human PHD-2/Egln1 protein.

Background

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).

1. Freeman, R.S. et al. (2003) Mol Cells 16, 1-12.
2. Villar, D. et al. (2007) Biochem J 408, 231-40.
3. Fu, J. et al. (2007) J Biol Chem 282, 12410-8.
4. D'Angelo, G. et al. (2003) J Biol Chem 278, 38183-7.
5. del Peso, L. et al. (2003) J Biol Chem 278, 48690-5.
6. Pescador, N. et al. (2005) Biochem J 390, 189-97.
7. Metzen, E. et al. (2005) Biochem J 387, 711-7.
8. Hirsilä, M. et al. (2003) J Biol Chem 278, 30772-80.
9. Mikhaylova, O. et al. (2008) Mol Cell Biol 28, 2701-17.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at -20°C. Do not aliquot the antibody.