Calmodulin Antibody

• 产品编号：CST-4830S      品牌：CST       原厂货号：4830S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Calmodulin（钙调蛋白）抗体识别内源性的全部钙调蛋白。该多克隆抗体用与人类钙调蛋白中Glu140附近氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化钙调蛋白是一个广泛表达的小蛋白，介导许多细胞作用，如短期和长期记忆，神经生长，炎症，细胞凋亡，肌肉收缩和细胞内运动（1）。与Ca2+结合后，钙调蛋白经过构象变化，以使复合物可以结合和激活许多酶，包括蛋白激酶、蛋白磷酸酶、离子通道、钙泵、一氧化氮合酶、三磷酸肌醇激酶和环核苷酸磷酸二酯酶（2,3）。由于钙调蛋白以协同方式结合Ca2+，细胞内Ca2+水平的微小变化会导致活性钙调蛋白及其靶蛋白水平的大幅变化（4）。

1. Persechini, A. et al. (1989) Trends Neurosci 12, 462-7.
2. Finn, B.E. et al. (1995) Nat Struct Biol 2, 777-83.
3. Agell, N. et al. (2002) Cell Signal 14, 649-54.
4. Porter, J.A. et al. (1993) Science 262, 1038-42.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Monkey, Xenopus, Pig

Specificity / Sensitivity

Calmodulin Antibody detects endogenous levels of total calmodulin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Glu140 of human calmodulin. Antibodies are purified by peptide affinity chromatography.

Background

Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement (1). Upon binding of four Ca2+ ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca2+ pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase (2,3). Since calmodulin binds Ca2+ in a cooperative fashion, small changes in cytosolic Ca2+ levels lead to large changes in the level of active calmodulin and its target proteins (4).

1. Persechini, A. et al. (1989) Trends Neurosci 12, 462-7.
2. Finn, B.E. et al. (1995) Nat Struct Biol 2, 777-83.
3. Agell, N. et al. (2002) Cell Signal 14, 649-54.
4. Porter, J.A. et al. (1993) Science 262, 1038-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.