Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb

• 产品编号：CST-4805S      品牌：CST       原厂货号：4805S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody 能够检测内源性957位丝氨酸磷酸化的SMC1蛋白水平。该抗体也可识别磷酸化的人DNA-PKcs (450kDa)。 该单克隆抗体是由合成的针对人SMC1的957位丝氨酸的磷酸化肽段免疫动物生产的。染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员，该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分，ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中，SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的，但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。

1. Michaelis, C. et al. (1997) Cell 91, 35-45.
2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957. This antibody may also recognize phosphorylated human DNA-PKcs (450kDa).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. 

Background

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

1. Michaelis, C. et al. (1997) Cell 91, 35-45.
2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody.