Phospho-SMC1 (Ser957) Antibody

• 产品编号：CST-4801S      品牌：CST       原厂货号：4801S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SMC1 (Ser957)抗体能够检测到957位丝氨酸磷酸化后的内源性SMC1蛋白。多抗由合成肽段免疫动物产生，该肽段与人SMC1蛋白957位丝氨酸附近氨基酸一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Structural maintenance of chromosomes 1 (SMC1)蛋白是一种染色体蛋白，属于黏连蛋白复合体，能够促进姐妹染色体黏附并在DNA修复过程中发挥作用（1,2）。SMC1的957和966位丝氨酸磷酸化依赖ATM/NBS1发生在intra-S-期DAN损伤检验点，是对电离辐射（IR）的应激反应（3）。SMC1不依赖于ATM的磷酸化发生于细胞出现其他DNA损伤时，包括UV和羟基脲处理（4）。IR引发的intra-S-期DNA损伤检验点需要SMC1磷酸化才能进行，但是该机制尚未完全清楚，可能包括了一些构象变化影响了SMC1-SMC3的相互作用（3）。

1. Michaelis, C. et al. (1997) Cell 91, 35-45.
2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Bovine

Specificity / Sensitivity

Phospho-SMC1 (Ser957) Antibody detects endogenous levels of SMC1 only when phosphorylated at Ser957.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

1. Michaelis, C. et al. (1997) Cell 91, 35-45.
2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody