Clathrin Heavy Chain (D3C6) XP? Rabbit mAb

• 产品编号：CST-4796P      品牌：Cell Signaling Technology       原厂货号：4796P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Clathrin Heavy Chain (D3C6) XP®Rabbit mAb兔单抗识别内源性的clathrin总蛋白。该单克隆抗体用与人类clathrin蛋白重链中Pro1663位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。包被Clathrin的囊泡通过内吞和多条囊泡运输途径介导细胞间蛋白转运。在细胞膜上囊泡形成的特异区域，Clathrin与相关蛋白结合形成Clathrin包被小凹。这些细胞膜相关的小凹内陷，内化蛋白质形成细胞内的Clathrin包被囊泡（1,2）。Clathrin在这些囊泡中大量存在，以三脚蛋白复合体的基本组装形式存在。每个Clathrin三脚蛋白复合体由三个clathrin重链和三个clathrin轻链组成。Clathrin重链蛋白质由多个功能域组成，包括一个羧基末端的可以与其它triskelion蛋白重链相互作用的区域，和一个球状的氨基末端可以与其它囊泡蛋白结合的区域（2）。接头蛋白如AP2、epsin和EPS15，负责将囊泡蛋白招募到小凹形成位点，以及集合clathrin包被的囊泡。囊泡内陷后，GTP酶作为动力收紧新生囊泡颈，形成完全自由的细胞内囊泡（3，4）。

1. Rodriguez-Boulan, E. et al. (2005) Nat. Rev. Mol. Cell Biol. 6, 233-247.
2. Mousavi, S.A. et al. (2004) Biochem. J. 377, 1-16.
3. Rappoport, J.Z. et al. (2004) Traffic 5, 327-237.
4. Brett, T.J. and Traub, L.M. (2006) Curr. Opin. Cell Biol. 18, 395-406.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

Clathrin Heavy Chain (D3C6) XP® Rabbit mAb detects endogenous levels of total clathrin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1663 of human clathrin heavy chain protein.

Background

Clathrin-coated vesicles provide for the intracellular transport of cargo proteins following endocytosis and during multiple vesicle trafficking pathways. Vesicles form at specialized areas of the cell membrane where clathrin and associated proteins form clathrin-coated pits. Invagination of these cell membrane-associated pits internalizes proteins and forms an intracellular clathrin-coated vesicle (1,2). Clathrin is the most abundant protein in these vesicles and is present as a basic assembly unit called a triskelion. Each clathrin triskelion is composed of three clathrin heavy chains and three clathrin light chains. Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins (2). Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle. Following vesicle invagination, the GTPase dynamin constricts the neck of the nascent vesicle to complete formation of the free, cytosolic vesicle (3,4).

1. Rodriguez-Boulan, E. et al. (2005) Nat. Rev. Mol. Cell Biol. 6, 233-247.
2. Mousavi, S.A. et al. (2004) Biochem. J. 377, 1-16.
3. Rappoport, J.Z. et al. (2004) Traffic 5, 327-237.
4. Brett, T.J. and Traub, L.M. (2006) Curr. Opin. Cell Biol. 18, 395-406.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.