CDC37 (D11A3) XP? Rabbit mAb

• 产品编号：CST-4793P      品牌：Cell Signaling Technology       原厂货号：4793P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDC37 (D11A3) XP?Rabbit mAb识别内源性的CDC37总蛋白。该单克隆抗体用与人类CDC37蛋白中Val297位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。CDC37是HSP90分子伴侣的重要组分（1，2）。它最初因参与细胞周期进程而被发现，后来发现它作为众多激酶和其它蛋白的分子伴侣而发挥了更广的多的作用（1-3）。CDC37蛋白有一个氨基末端激酶结合域，其后是一个中央HSP90结合域。它能够在HSP90复合物中招募和稳定激酶，保护新生合成激酶肽段不被降解，促进进入蛋白成熟的下一阶段（4，5）。CDC37也能抑制HSP90的ATP酶活性，从而导致复合物构象改变以排除目标激酶的装载（6）。CDC37因其在多个涉及增生和肿瘤细胞生存的激酶信号通路如Raf、Akt、Src和ErbB2扮演重要角色，所以认为其是潜在的治疗靶点（7，8）。

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

原厂资料：

Specificity / Sensitivity

CDC37 (D11A3) XP® Rabbit mAb detects endogenous levels of total CDC37 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val297 of human CDC37 protein.

Background

CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell-cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, and ErbB2 pathways (7,8).

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody