Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody

• 产品编号：CST-4771S      品牌：CST       原厂货号：4771S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody分别检测仅在lysine 1535或lysine 1499位点乙酰化的内源性CBP或p300蛋白水平（亚型1）。通过合成的与人源CBP蛋白Lys1535位点周围相应的乙酰化多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。CBP (CREB-binding protein)和p300蛋白是高度保守的并且具有与转录共激活因子有关联的功能，它是与转录调节因子和信号分子有关联，使用转录分子去整合多种信号转导通路(1,2)。CBP/p300也包含组蛋白乙酰转移酶(HAT)活性，它允许它们使组蛋白或其它蛋白发生乙酰化(2)。通过PKC使p300蛋白在Ser89位点的磷酸化抑制它的转录活性，并且通过AMPK使相同位点的磷酸化干扰了细胞核受体与p300蛋白的联系(3,4)。Akt使p300蛋白Ser1834位点的磷酸化干扰它与C/EBPβ的联系(5)。生长因子可诱导CBP在Ser437位点磷酸化，这对于CBP被招募到转录复合物上时需要的(6)。CaM激酶IV 使CBP蛋白的Ser302位点磷酸化，这在中枢神经系统中对于CBP依赖的转录活性是需要的(7)。人们对CBP/p300蛋白乙酰化的作用很感兴趣(2,8)。p300蛋白Lys1499位点的乙酰化已经被证明能提高它的HAT活性，并且影响很多信号传导(9)。

1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
7. Impey, S. et al. (2002) Neuron 34, 235-244.
8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.
10. Stiehl, D.P. et al. (2007) Cancer Res 67, 2256-64.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody detects endogenous levels of CBP or p300 only when acetylated at lysine 1535 or lysine 1499, respectively.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of Lys1499 of p300 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).

1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
7. Impey, S. et al. (2002) Neuron 34, 235-244.
8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.
10. Stiehl, D.P. et al. (2007) Cancer Res 67, 2256-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.